Product Info Summary
SKU: | A10395-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-MRP-L12 Antibody
SKU/Catalog Number
A10395-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRP-L12 Antibody catalog # A10395-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-L12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10395-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human MRP-L12. at AA rangle: 1-80
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10395-1 is reactive to MRPL12 in Human, Mouse
Applications
A10395-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21348 MW
Background of MRPL12
Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells). Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines. Anti-Cybr Antibody is suitable for researchers interested in the Research Areas of Chromatin, Immunology, Neuroscience, and Signal Transduction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
![a10395 1 western blotting analysis 1 a10395 1 western blotting analysis 1](https://www.bosterbio.com/media/catalog/product/a/1/a10395-1-western-blotting-analysis-1.jpg)
Click image to see more details
Western Blot (WB) analysis of specific cells using MRP-L12 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For MRPL12 (Source: Uniprot.org, NCBI)
Gene Name
MRPL12
Full Name
39S ribosomal protein L12, mitochondrial
Weight
21348 MW
Superfamily
bacterial ribosomal protein bL12 family
Alternative Names
5c5-2; FLJ60124; L12mt; MGC8610; mitochondrial ribosomal protein L12; MRP-L12; MRP-L31/34; MRPL7; MRPL7/L12; RPML1239S ribosomal protein L12, mitochondrial MRPL12 5c5-2, L12mt, MRP-L31/34, MRPL7, MRPL7/L12, RPML12 mitochondrial ribosomal protein L12 39S ribosomal protein L12, mitochondrial|MRP-L12|mitochondrial large ribosomal subunit protein bL12m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL12, check out the MRPL12 Infographic
![MRPL12 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP-L12 Antibody (A10395-1)
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No publications found for A10395-1
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Customer Q&As
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7 Customer Q&As for Anti-MRP-L12 Antibody
Question
Does A10395-1 anti-MRP-L12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
It shows on the product datasheet, A10395-1 anti-MRP-L12 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for apex of heart using anti-MRP-L12 antibody A10395-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-11
Question
I see that the anti-MRP-L12 antibody A10395-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-08
Question
Would anti-MRP-L12 antibody A10395-1 work on pig WB with muscle?
P. Jackson
Verified customer
Asked: 2018-05-08
Answer
Our lab technicians have not validated anti-MRP-L12 antibody A10395-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MRP-L12 antibody A10395-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig muscle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-08
Question
We are currently using anti-MRP-L12 antibody A10395-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
The anti-MRP-L12 antibody (A10395-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-24
Question
Is this A10395-1 anti-MRP-L12 antibody reactive to the isotypes of MRPL12?
D. Roberts
Verified customer
Asked: 2016-06-13
Answer
The immunogen of A10395-1 anti-MRP-L12 antibody is Synthesized peptide derived from the Internal region of human MRP-L12. at AA rangle: 1-80. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-06-13
Question
I was wanting to use your anti-MRP-L12 antibody for WB for mouse apex of heart on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse apex of heart identification?
R. Huang
Verified customer
Asked: 2014-12-17
Answer
It shows on the product datasheet, A10395-1 anti-MRP-L12 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse apex of heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-17