Product Info Summary
SKU: | M00500 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, WB |
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Product info
Product Name
Anti-SNF5 SMARCB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00500
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SNF5 SMARCB1 Rabbit Monoclonal Antibody catalog # M00500. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SNF5 SMARCB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00500)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFO-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SNF5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00500 is reactive to SMARCB1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
44141 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00500 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: HeLa cell, K562 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SNF5 in (1) HeLa cell lysate; (2) K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For SMARCB1 (Source: Uniprot.org, NCBI)
Gene Name
SMARCB1
Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
Weight
44141 MW
Superfamily
SNF5 family
Alternative Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1;BRG1-associated factor 47;BAF47;Integrase interactor 1 protein;SNF5 homolog;hSNF5;SMARCB1;BAF47, INI1, SNF5L1; SMARCB1 BAF47, CSS3, INI1, MRD15, PPP1R144, RDT, RTPS1, SNF5, SNF5L1, SWNTS1, Sfh1p, Snr1, hSNFS SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1|BRG1-associated factor 47|SNF5 homolog|SWI/SNF-related matrix-associated protein|hSNF5|integrase interactor 1 protein|malignant rhabdoid tumor suppressor|protein phosphatase 1, regulatory subunit 144|sucrose nonfermenting, yeast, homolog-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMARCB1, check out the SMARCB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMARCB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SNF5 SMARCB1 Rabbit Monoclonal Antibody (M00500)
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No publications found for M00500
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Customer Q&As
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4 Customer Q&As for Anti-SNF5 SMARCB1 Rabbit Monoclonal Antibody
Question
I am looking for to test anti-SNF5 Rabbit Monoclonal antibody M00500 on mouse embryonic kidney for research purposes, then I may be interested in using anti-SNF5 Rabbit Monoclonal antibody M00500 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The products we sell, including anti-SNF5 Rabbit Monoclonal antibody M00500, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-26
Question
Is a blocking peptide available for product anti-SNF5 Rabbit Monoclonal antibody (M00500)?
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
We do provide the blocking peptide for product anti-SNF5 Rabbit Monoclonal antibody (M00500). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-12
Question
We are currently using anti-SNF5 Rabbit Monoclonal antibody M00500 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
The anti-SNF5 Rabbit Monoclonal antibody (M00500) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-SNF5 Rabbit Monoclonal antibody M00500. Let me know if you need anything else.
L. Miller
Verified customer
Asked: 2013-02-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-26