Product Info Summary
SKU: | A30616 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-CLDN6/Claudin 6 Antibody
SKU/Catalog Number
A30616
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CLDN6/Claudin 6 Antibody (Catalog# A30616). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLDN6/Claudin 6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30616)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CLDN6. Immunogen sequence location: 81-130
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30616 is reactive to CLDN6 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
23292 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30616 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using CLDN6 Antibody. The picture on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of HUVEC cells, using CLDN6 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CLDN6 (Source: Uniprot.org, NCBI)
Gene Name
CLDN6
Full Name
Claudin-6
Weight
23292 MW
Superfamily
claudin family
Alternative Names
CLDN6;Claudin-6;Skullin CLDN6 claudin 6 claudin-6|skullin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLDN6, check out the CLDN6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLDN6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLDN6/Claudin 6 Antibody (A30616)
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No publications found for A30616
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CLDN6/Claudin 6 Antibody
Question
Our team were well pleased with the WB result of your anti-CLDN6/Claudin 6 antibody. However we have observed positive staining in placenta cell junction using this antibody. Is that expected? Could you tell me where is CLDN6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
According to literature, placenta does express CLDN6. Generally CLDN6 expresses in cell junction, tight junction. Regarding which tissues have CLDN6 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-28
Question
you antibody using your anti-CLDN6/Claudin 6 antibody for cell-cell junction organization studies. Has this antibody been tested with western blotting on huvec cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
I appreciate your inquiry. This A30616 anti-CLDN6/Claudin 6 antibody is tested on human ovary tissue, huvec cells. It is guaranteed to work for IF, IHC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-07
Question
We are currently using anti-CLDN6/Claudin 6 antibody A30616 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-22
Answer
The anti-CLDN6/Claudin 6 antibody (A30616) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-22
Question
We have been able to see staining in human placenta. Are there any suggestions? Is anti-CLDN6/Claudin 6 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
From what I have seen in literature placenta does express CLDN6. From what I have seen in Uniprot.org, CLDN6 is expressed in female gonad, placenta, among other tissues. Regarding which tissues have CLDN6 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-08-28
Question
We ordered your anti-CLDN6/Claudin 6 antibody for IF on placenta last year. I am using human, and We are going to use the antibody for IHC next. We are interested in examining placenta as well as female gonad in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
A. Jha
Verified customer
Asked: 2016-01-26
Answer
I looked at the website and datasheets of our anti-CLDN6/Claudin 6 antibody and it seems that A30616 has been tested on human in both IF and IHC. Thus A30616 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-01-26