Anti-SMYD3 Antibody

Product Name

Anti-SMYD3 Antibody

View all SMYD3 Antibodies

SKU/Catalog Number

A02080-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-SMYD3 Antibody catalog # A02080-1. Tested in WB,ICC/IF,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMYD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02080-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human SP7 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02080-1 is reactive to SMYD3 in Human, Mouse, Rat

Applications

A02080-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

49097 MW

Background of SMYD3

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.

McKinnon C.M., Eur. J. Biochem. 169:547-553(1987).
Tosi M., Biochemistry 26:8516-8524(1987).
Kusumoto H., Proc. Natl. Acad. Sci. U.S.A. 85:7307-7311(1988).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2,000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of SMYD3 using Anti-SMYD3 Antibody (A02080-1).

ICC staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-SMYD3 Antibody A02080-1

Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1

Gene/Protein Information For SMYD3 (Source: Uniprot.org, NCBI)

Alternative Names

bA74P14.1 (novel protein); bA74P14.1; EC 2.1.1; EC 2.1.1.43; FLJ21080; KMT3E; MGC104324; MYND domain containing 1; SET and MYND domain containing 3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; ZMYND1; ZNFN3A1 SMYD3 KMT3E, ZMYND1, ZNFN3A1, bA74P14.1 SET and MYND domain containing 3 histone-lysine N-methyltransferase SMYD3|SET and MYND domain-containing protein 3|zinc finger MYND domain-containing protein 1|zinc finger protein, subfamily 3A (MYND domain containing), 1|zinc finger, MYND domain containing 1

For more info on SMYD3, check out the SMYD3 Infographic

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In this infographic, you will see the following information for SMYD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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