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Product Info Summary
| SKU: | PB9893 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SMYD3 Antibody Picoband®
SKU/Catalog Number
PB9893
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SMYD3 Antibody Picoband® catalog # PB9893. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMYD3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9893)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SMYD3, different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9893 is reactive to SMYD3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
49097 MW
Background of SMYD3
SET and MYND domain-containing protein 3 is a protein that in humans is encoded by the SMYD3 gene. The International Radiation Hybrid Mapping Consortium mapped the SMYD3 gene to chromosome 1. This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9893 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole cell, COLO320 whole cell
IHC: human intestinal cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SMYD3 using anti-SMYD3 antibody (PB9893).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMYD3 antigen affinity purified polyclonal antibody (Catalog # PB9893) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMYD3 at approximately 55 kDa. The expected band size for SMYD3 is at 49 kDa.
Click image to see more details
Figure 2. IHC analysis of SMYD3 using anti-SMYD3 antibody (PB9893).
SMYD3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SMYD3 Antibody (PB9893) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SMYD3 (Source: Uniprot.org, NCBI)
Gene Name
SMYD3
Full Name
Histone-lysine N-methyltransferase SMYD3
Weight
49097 MW
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
Histone-lysine N-methyltransferase SMYD3;2.1.1.43;SET and MYND domain-containing protein 3;Zinc finger MYND domain-containing protein 1;SMYD3;ZMYND1, ZNFN3A1; SMYD3 KMT3E, ZMYND1, ZNFN3A1, bA74P14.1 SET and MYND domain containing 3 histone-lysine N-methyltransferase SMYD3|SET and MYND domain-containing protein 3|zinc finger MYND domain-containing protein 1|zinc finger protein, subfamily 3A (MYND domain containing), 1|zinc finger, MYND domain containing 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMYD3, check out the SMYD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMYD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMYD3 Antibody Picoband® (PB9893)
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1 Customer Q&As for Anti-SMYD3 Antibody Picoband®
Question
We are currently using anti-SMYD3 antibody PB9893 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
E. Brown
Verified customer
Asked: 2017-08-24
Answer
The anti-SMYD3 antibody (PB9893) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-24
