Anti-HDAC7 Antibody Picoband™

Product Name

Anti-HDAC7 Antibody Picoband™

View all HDAC7 Antibodies

SKU/Catalog Number

PB9629

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HDAC7 Antibody Picoband™ catalog # PB9629. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HDAC7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9629)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HDAC7, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9629 is reactive to HDAC7 in Human, Rat

Applications

PB9629 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

103 kDa

Calculated molecular weight

102927 MW

Background of HDAC7

Histone deacetylase 7 is an enzyme that in humans is encoded by the HDAC7 gene. It is mapped to 12q13.1. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation / deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HDAC7 using anti-HDAC7 antibody (PB9629).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Spleen Tissue Lysate at 50ug,
Lane 2: Rat Thymus Tissue Lysate at 50ug,
Lane 3: Human Placenta Tissue Lysate at 50ug,
Lane 4: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC7 antigen affinity purified polyclonal antibody (Catalog # PB9629) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC7 at approximately 103 kDa. The expected band size for HDAC7 is at 103 kDa.

Gene/Protein Information For HDAC7 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZP586J0917; EC 3.5.1.98; HD7; HD7A; HDAC7AFLJ99588; histone deacetylase 7; Histone deacetylase 7ADKFZp586J0917 HDAC7 HD7, HD7AA, HDAC7 histone deacetylase 7 histone deacetylase 7|histone deacetylase 7A

For more info on HDAC7, check out the HDAC7 Infographic

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In this infographic, you will see the following information for HDAC7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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