Product Info Summary
SKU: | A00784 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Smad7 Antibody
SKU/Catalog Number
A00784
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Smad7 Antibody catalog # A00784. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00784)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human GATA-6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00784 is reactive to SMAD7 in Human, Mouse, Rat
Applications
A00784 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
46426 MW
Background of SMAD7
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Ana V. Miletic, J. Biol. Chem., Dec 2006; 281: 38257 - 38265.
Christopher C. Stebbins, Mol. Cell. Biol., Sep 2003; 23: 6291 - 6299.
Vered Schapira, Cancer Res., Jun 2006; 66: 6183 - 6191.
Maya Groysman, J. Biol. Chem., Dec 2002; 277: 50121 - 50130.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Smad7 Antibody A00784
Western blot (WB) analysis of Smad7 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate
Lane2:sp2/0 whole cell lysate
Lane3:PC12 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SMAD7 (Source: Uniprot.org, NCBI)
Gene Name
SMAD7
Full Name
Mothers against decapentaplegic homolog 7
Weight
46426 MW
Superfamily
dwarfin/SMAD family
Alternative Names
CRCS3; FLJ16482; hSMAD7; MAD homolog 7; MAD homolog 8; MAD, mothers against decapentaplegic homolog 7 (Drosophila); MADH7; MADH7mothers against decapentaplegic homolog 7; MADH8; Mothers against decapentaplegic homolog 8; Mothers against decapentaplegic, drosophila, homolog of, 7; Mothers against DPP homolog 7; Mothers against DPP homolog 8; SMAD 7; SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7; SMAD, mothers against DPP homolog 7 (Drosophila); SMAD, mothers against DPP homolog 7; Smad7 SMAD7 CRCS3, MADH7, MADH8 SMAD family member 7 mothers against decapentaplegic homolog 7|MAD (mothers against decapentaplegic, Drosophila) homolog 7|MAD homolog 8|Mothers against decapentaplegic, drosophila, homolog of, 7|SMAD, mothers against DPP homolog 7|hSMAD7|mothers against DPP homolog 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD7, check out the SMAD7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad7 Antibody (A00784)
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No publications found for A00784
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