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Product Info Summary
| SKU: | A05830-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SLAMF3 LY9 Antibody
View all CD229/SLAMF3/Lymphocyte Antigen 9 Antibodies
SKU/Catalog Number
A05830-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SLAMF3 LY9 Antibody (Catalog # A05830-2). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SLAMF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SLAMF3 LY9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05830-2)
Host
Rabbit
Contents
SLAMF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SLAMF3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SLAMF3. The immunogen is located within amino acids 520 - 570 of SLAMF3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of SLAMF3 are known to exist. SLAMF3 antibody is predicted to not cross-react with other SLAM protein family members.
Reactive Species
A05830-2 is reactive to LY9 in Human, Mouse, Rat
Applications
A05830-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
72139 MW
Background of CD229/SLAMF3/Lymphocyte Antigen 9
The signaling lymphocyte-activation molecule family member 3 (SLAMF3), also known as LY9, is a cell surface receptor that is expressed on T and B lymphocytes and belongs to the CD150/SLAMF1 receptor family. SLAMF3 was identified through a yeast two-hybrid screening in which SLAMF3 bound to the X-linked lymphoproliferative disease gene product SAP, suggesting that in activated T cells, SLAMF3 signaling can be triggered SAP. Its expression on the cell surface appears to be regulated via its interaction with the clathrin-associated adaptor complex 2 (AP-2). Despite its similarity to SLAMF1 in structure and interactions with SAP, SLAMF3-deficient mice do not exhibit phenotypic characteristics associated with SLAMF1- and SAP-deficient mice, suggesting that SLAMF3 plays other roles in T cell activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SLAMF3 antibody can be used for detection of SLAMF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SLAMF3 in 293 cell lysate with SLAMF3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of SLAMF3 in 293 cells with SLAMF3 antibody at 10 μg/mL.
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Immunofluorescence of SLAMF3 in 293 cells with SLAMF3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For LY9 (Source: Uniprot.org, NCBI)
Gene Name
LY9
Full Name
T-lymphocyte surface antigen Ly-9
Weight
72139 MW
Alternative Names
CD229 antigen; CD229; cell-surface molecule Ly-9; hly9; Ly9; lymphocyte antigen 9Cell surface molecule Ly-9; mLY9; SLAMF3; T-lymphocyte surface antigen Ly-9 LY9 CD229, SLAMF3, hly9, mLY9 lymphocyte antigen 9 T-lymphocyte surface antigen Ly-9|SLAM family member 3|cell surface molecule Ly-9|signaling lymphocytic activation molecule 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LY9, check out the LY9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LY9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLAMF3 LY9 Antibody (A05830-2)
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