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Product Info Summary
| SKU: | PB9324 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NF-kB p65/RELA Antibody Picoband®
SKU/Catalog Number
PB9324
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NF-kB p65/RELA Antibody Picoband® catalog # PB9324. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF-kB p65/RELA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9324)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse NF-kB p65 recombinant protein (Position: D291-Q479). Mouse NF-kB p65 shares 77% amino acid (aa) sequence identity with human NF-kB p65.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9324 is reactive to Rela in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
60212 MW
Background of RELA
Transcription factor p65, also known as NFKB3 or NF-kB p65, is a protein encoded by the RELA gene. NFKB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc, and it may play a role in inflammatory conditions of the peripheral nervous system. Phosphorylation and acetylation of NFKB3 are crucial post-translational modifications required for NFKB activation. It has also been shown to modulate immune responses, and activation of NFKB3 is positively associated with multiple types of cancer. In addition to that, NFKB3 antagonizes TNFR1-JNK proliferative signals in epidermis and plays a nonredundant role in restraining epidermal growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9324 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: mouse NIH/3T3 whole cell, mouse ANA-1 whole cellrat testis tissuerat PC-12 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody (PB9324).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse NIH/3T3 whole cell lysates,
Lane 2: mouse ANA-1 whole cell lysates.
Lane 3: rat testis tissue lysates.
Lane 4: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65/RELA antigen affinity purified polyclonal antibody (Catalog # PB9324) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65/RELA at approximately 65 kDa. The expected band size for NF-kB p65/RELA is at 60 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Rela (Source: Uniprot.org, NCBI)
Gene Name
Rela
Full Name
Transcription factor p65
Weight
60212 MW
Alternative Names
Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;Rela;Nfkb3; RELA CMCU, NFKB3, p65 RELA proto-oncogene, NF-kB subunit transcription factor p65|NF-kappa-B p65delta3|NF-kappa-B transcription factor p65|nuclear factor NF-kappa-B p65 subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Rela, check out the Rela Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Rela: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NF-kB p65/RELA Antibody Picoband® (PB9324)
Hello CJ!
PB9324 has been cited in 54 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xin X,Yao D,Zhang K,Han S,Liu D,Wang H,Liu X,Li G,Huang J,Wang J.Protective effects of Rosavin on bleomycin-induced pulmonary fibrosis via suppressing fibrotic and inflammatory signaling pathways in mice.Biomed Pharmacother.2019 Jul;115:108870.doi:10.1016/j.biopha.2019.108870.Epub 2019 Apr 23.PMID:31026730.
Species: Mouse
PB9324 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:500
Li HN,Yang QQ,Wang WT,Tian X,Feng F,Zhang ST,Xia YT,Wang JX,Zou YW,Wang JY,Zeng XY.Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.J Neuroinflammation.2021 Jul 5;18(1):150.doi:10.1186/s12974-021-02198-9.PMID:34225736;PMCID:PMC8258957.
Species: Rat
PB9324 usage in article: APP:WB, SAMPLE:RN TISSUE, DILUTION:1:400
Lv Q,Xia Q,Li A,Wang Z. The Potential Role of IL1RAP on Tumor Microenvironment-Related Inflammatory Factors in Stomach Adenocarcinoma. Technol Cancer Res Treat. 2021 Jan-Dec;20: 1533033821995282.doi:10.1177/1533033821995282. PMID: 33602046.
Species: Human,Mouse
PB9324 usage in article: APP:WB, SAMPLE:MGC803 CELL, DILUTION:NA
Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
PB9324 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
PB9324 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
Species: Mouse
PB9324 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
Wang Y,Yang Z,Yang L,Zou Q,Zhao S,Hu N,Chen D,Cui R,Ma H.Liuweidihuang Pill Alleviates Inflammation of the Testis via AMPK/SIRT1/NF-κB Pathway in Aging Rats.Evid Based Complement Alternat Med.2020 May 24;2020:2792738.doi:10.1155/2020/2792738.PMID:32565851
Species: Rat
PB9324 usage in article: APP:WB, SAMPLE:TESTICULAR TISSUE, DILUTION:NA
Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
Wei JL,Wu CJ,Chen JJ,Shang FT,Guo SG,Zhang XC,Liu H.LncRNA NEAT1 promotes the progression of sepsis-induced myocardial cell injury by sponging miR-144-3p.Eur Rev Med Pharmacol Sci.2020 Jan;24(2):851-861.doi:10.26355/eurrev_202001_20069.PMID:32016991.
Species: Mouse
PB9324 usage in article: APP:WB, SAMPLE:HL-1 CELLS, DILUTION:1:1000
Nuclear factor-kappaB activation on the reactive oxygen species in acute necrotizing pancreatitic rats
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-NF-kB p65/RELA Antibody Picoband®
Question
We are currently using anti-NF-kB p65/RELA antibody PB9324 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on pig tissues as well?
J. Li
Verified customer
Asked: 2014-04-21
Answer
The anti-NF-kB p65/RELA antibody (PB9324) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-21
