Click image to see more details
Product Info Summary
| SKU: | EK1932 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human LY9 ELISA Kit PicoKine®
View all CD229/SLAMF3/Lymphocyte Antigen 9 ELISA kits
SKU/Catalog Number
EK1932
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LY9 ELISA Kit PicoKine® (96 Tests). Quantitate Human LY9 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LY9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1932)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: K48-K454
Sensitivity
<15 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1932 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1932 is reactive to LY9 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1932 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD229/SLAMF3/Lymphocyte Antigen 9
T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 17q21.31. LY9 has also recently been designated CD229. LY9 belongs to the SLAM family of immunomodulatory receptors and interacts with the adaptor molecule SAP.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1932-CAP | Anti-Human LY9 Pre-coated 96-well strip microplate | 1 |
| EK1932-ST | Human LY9 Standard | 2 vials, 10 ng/tube |
| EK1932-DA | Human LY9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.067 | 0.154 | 0.192 | 0.323 | 0.492 | 0.732 | 1.391 | 2.245 |
Data Example Images
Click image to see more details
Human LY9 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD229/SLAMF3/Lymphocyte Antigen 9, Dilution ratio of 1:1, concentration in serum and plasma is around 1000 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LY9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 92 | 698 | 2164 | 108 | 736 | 2366 |
| Standard deviation | 5.43 | 30.01 | 110.36 | 7.13 | 42.69 | 108.84 |
| CV (%) | 5.9% | 4.3% | 5.1% | 6.6% | 5.8% | 4.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD229/SLAMF3/Lymphocyte Antigen 9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 92 | 93 | 98 | 92 | 93 | 2.48 | 2.6% |
| Sample 2 | 698 | 622 | 636 | 644 | 650 | 28.80 | 4.4% |
| Sample 3 | 2164 | 1927 | 1955 | 2266 | 2078 | 142.01 | 6.8% |
Protein Target Info & Infographic
Gene/Protein Information For LY9 (Source: Uniprot.Org, NCBI)
Gene Name
LY9
Full Name
T-lymphocyte surface antigen Ly-9
Weight
72.139kDa
Alternative Names
T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD229; LY9; CDABP0070 LY9 CD229, SLAMF3, hly9, mLY9 lymphocyte 9 T-lymphocyte surface Ly-9|SLAM family member 3|cell surface molecule Ly-9|signaling lymphocytic activation molecule 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LY9, check out the LY9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LY9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human LY9 ELISA Kit PicoKine® (EK1932)
Hello CJ!
No publications found for EK1932
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human LY9 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human LY9 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human LY9 ELISA Kit PicoKine®
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
D. Mangal
Verified customer
Asked: 2020-11-09
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-11-09
Question
Q: how to thaw whole blood sample for CD229 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-05-27
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD229 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-05-27
Question
Q: how do I analyze ELISA data? I have obtained CD229 level in serum.
Verified Customer
Verified customer
Asked: 2019-11-10
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-10
Question
Q: is it okay to use citrate plasma as samples in Human CD229 Picokine® ELISA Kit (Catalog # EK1932)?
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CD229 causing degradation of its protein structure. CD229 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CD229 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-02-04
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CD229 using the EK1932 Human CD229 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-02-01
Question
Q: if the enzyme conjugated CD229 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD229 antigen?
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-08-21
Question
Q: can you recommend the dilution ratio of serum samples for detection of CD229 in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD229 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-06-06
