Click image to see more details
Product Info Summary
| SKU: | PA1616 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IL2 Receptor gamma/IL2RG Antibody Picoband®
View all Common gamma Chain/IL-2 R gamma Antibodies
SKU/Catalog Number
PA1616
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL2 Receptor gamma/IL2RG Antibody catalog # PA1616. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL2 Receptor gamma/IL2RG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1616)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human IL2 Receptor gamma.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1616 is reactive to IL2RG in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
42287 MW
Background of Common gamma Chain/IL-2 R gamma
IL2RG (INTERLEUKIN 2 RECEPTOR, GAMMA),also known as Common gamma chain or CD132, is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors:IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. IL2RG is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. By fluorescence in situ hybridization and PCR amplification of somatic cell hybrid DNAs, The IL2RG gene is mapped to Xq13.1. The IL2RG gene contains 8 exons and spans approximately 4.2 kb. And the gamma chain is an indispensable component of the functional IL2 receptor. Lymphocytes expressing the common gamma chain can form functional receptors for these cytokine proteins, which transmit signals from one cell to another and direct programs of cellular differentiation. The sharing of the gamma subunit by several receptors explains why humans and mice that lack IL2 entirely show milder symptoms than those with IL2RG deficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1616 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: PANC Cell, HELA Cell, JURKAT Cell, RAJI Cell, CEM Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-IL2 Receptor gamma antibody, PA1616, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: RAJI Cell Lysate
Lane 5: CEM Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For IL2RG (Source: Uniprot.org, NCBI)
Gene Name
IL2RG
Full Name
Cytokine receptor common subunit gamma
Weight
42287 MW
Superfamily
type I cytokine receptor family
Alternative Names
Cytokine receptor common subunit gamma;Interleukin-2 receptor subunit gamma;IL-2 receptor subunit gamma;IL-2R subunit gamma;IL-2RG;gammaC;p64;CD132;IL2RG; IL2RG CD132, CIDX, IL-2RG, IMD4, P64, SCIDX, SCIDX1 interleukin 2 receptor subunit gamma cytokine receptor common subunit gamma|CD132 |IL-2 receptor subunit gamma|IL-2R subunit gamma|common cytokine receptor gamma chain|gammaC|interleukin 2 receptor, gamma|nonfunctional common cytokine receptor gamma chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL2RG, check out the IL2RG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL2RG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL2 Receptor gamma/IL2RG Antibody Picoband® (PA1616)
Hello CJ!
No publications found for PA1616
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IL2 Receptor gamma/IL2RG Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IL2 Receptor gamma/IL2RG Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-IL2 Receptor gamma/IL2RG Antibody Picoband®
Question
We have observed staining in human leukemic t-cell. Any tips? Is anti-IL2 Receptor gamma/IL2RG antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
From what I have seen in literature leukemic t-cell does express IL2RG. From what I have seen in Uniprot.org, IL2RG is expressed in leukocyte, liver, b-cell, leukemic t-cell, among other tissues. Regarding which tissues have IL2RG expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 8514792, 8401490
Boster Scientific Support
Answered: 2020-02-14
Question
Is a blocking peptide available for product anti-IL2 Receptor gamma/IL2RG antibody (PA1616)?
H. Walker
Verified customer
Asked: 2019-10-10
Answer
We do provide the blocking peptide for product anti-IL2 Receptor gamma/IL2RG antibody (PA1616). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-10
Question
Is this PA1616 anti-IL2 Receptor gamma/IL2RG antibody reactive to the isotypes of IL2RG?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The immunogen of PA1616 anti-IL2 Receptor gamma/IL2RG antibody is A synthetic peptide corresponding to a sequence in the middle region of human IL2 Receptor gamma(88-102aa HYWYKNSDNDKVQKC). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-27
Question
Is there a BSA free version of anti-IL2 Receptor gamma/IL2RG antibody PA1616 available?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL2 Receptor gamma/IL2RG antibody PA1616 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-20
Question
I have a question about product PA1616, anti-IL2 Receptor gamma/IL2RG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1616 anti-IL2 Receptor gamma/IL2RG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-18
Question
I see that the anti-IL2 Receptor gamma/IL2RG antibody PA1616 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-24
Question
Does anti-IL2 Receptor gamma/IL2RG antibody PA1616 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
According to the expression profile of leukocyte, IL2RG is highly expressed in leukocyte. So, it is likely that anti-IL2 Receptor gamma/IL2RG antibody PA1616 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2019-02-12
Question
We are currently using anti-IL2 Receptor gamma/IL2RG antibody PA1616 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
The anti-IL2 Receptor gamma/IL2RG antibody (PA1616) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-18
Question
I was wanting to use your anti-IL2 Receptor gamma/IL2RG antibody for WB for human leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
It shows on the product datasheet, PA1616 anti-IL2 Receptor gamma/IL2RG antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-11
Question
We are interested in to test anti-IL2 Receptor gamma/IL2RG antibody PA1616 on human leukocyte for research purposes, then I may be interested in using anti-IL2 Receptor gamma/IL2RG antibody PA1616 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
The products we sell, including anti-IL2 Receptor gamma/IL2RG antibody PA1616, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-06
Question
Does anti-IL2 Receptor gamma/IL2RG antibody PA1616 work on canine WB with leukocyte?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
Our lab technicians have not tested anti-IL2 Receptor gamma/IL2RG antibody PA1616 on canine. You can run a BLAST between canine and the immunogen sequence of anti-IL2 Receptor gamma/IL2RG antibody PA1616 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine leukocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-29
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-IL2 Receptor gamma/IL2RG antibody PA1616. Let me know if you need anything else.
S. Roberts
Verified customer
Asked: 2015-09-23
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-23
Question
Would PA1616 anti-IL2 Receptor gamma/IL2RG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Li
Verified customer
Asked: 2015-03-06
Answer
As indicated on the product datasheet, PA1616 anti-IL2 Receptor gamma/IL2RG antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-06
Question
See attached the WB image, lot number and protocol we used for leukocyte using anti-IL2 Receptor gamma/IL2RG antibody PA1616. Please let me know if you require anything else.
G. Carter
Verified customer
Asked: 2014-10-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-20
Question
We were content with the WB result of your anti-IL2 Receptor gamma/IL2RG antibody. However we have observed positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is IL2RG supposed to be expressed?
M. Kulkarni
Verified customer
Asked: 2013-12-05
Answer
Based on literature, liver does express IL2RG. Generally IL2RG expresses in cell membrane. Regarding which tissues have IL2RG expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 8514792, 8401490
Boster Scientific Support
Answered: 2013-12-05
Question
I am looking for using your anti-IL2 Receptor gamma/IL2RG antibody for immune response studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
J. Anderson
Verified customer
Asked: 2013-05-27
Answer
We appreciate your inquiry. This PA1616 anti-IL2 Receptor gamma/IL2RG antibody is validated on panc cell lysate, hela cell lysate, jurkat cell lysate, raji cell lysate, cem cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-05-27
