Product Info Summary
SKU: | M03358 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-SHIP INPP5D Rabbit Monoclonal Antibody
SKU/Catalog Number
M03358
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SHIP INPP5D Rabbit Monoclonal Antibody catalog # M03358. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHIP INPP5D Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03358)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AHG-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SHIP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03358 is reactive to INPP5D in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
87 kDa
Calculated molecular weight
133292 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03358 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: Daudi cell
IHC: rat spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SHIP1 expression in Daudi cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded rat spleen, using SHIP Antibody.
Protein Target Info & Infographic
Gene/Protein Information For INPP5D (Source: Uniprot.org, NCBI)
Gene Name
INPP5D
Full Name
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Weight
133292 MW
Superfamily
inositol 1,4,5-trisphosphate 5-phosphatase family
Alternative Names
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1;3.1.3.86;Inositol polyphosphate-5-phosphatase of 145 kDa;SIP-145;SH2 domain-containing inositol 5'-phosphatase 1;SH2 domain-containing inositol phosphatase 1;SHIP-1;p150Ship;hp51CN;INPP5D;SHIP, SHIP1; INPP5D SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship inositol polyphosphate-5-phosphatase D phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1|SH2 domain-containing inositol 5-phosphatase 1|inositol polyphosphate-5-phosphatase, 145kD|inositol polyphosphate-5-phosphatase, 145kDa|phosphatidylinositol 4,5-bisphosphate 5-phosphatase|signaling inositol polyphosphate 5 phosphatase SIP-145|signaling inositol polyphosphate phosphatase SHIP II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INPP5D, check out the INPP5D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INPP5D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHIP INPP5D Rabbit Monoclonal Antibody (M03358)
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No publications found for M03358
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Customer Q&As
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5 Customer Q&As for Anti-SHIP INPP5D Rabbit Monoclonal Antibody
Question
Will anti-SHIP Rabbit Monoclonal antibody M03358 work on dog IP with b-cell lymph?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
Our lab technicians have not tested anti-SHIP Rabbit Monoclonal antibody M03358 on dog. You can run a BLAST between dog and the immunogen sequence of anti-SHIP Rabbit Monoclonal antibody M03358 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog b-cell lymph in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-SHIP Rabbit Monoclonal antibody M03358. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-17
Question
Will anti-SHIP Rabbit Monoclonal antibody M03358 work for WB with lung?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
According to the expression profile of lung, INPP5D is highly expressed in lung. So, it is likely that anti-SHIP Rabbit Monoclonal antibody M03358 will work for WB with lung.
Boster Scientific Support
Answered: 2017-06-20
Question
Is this M03358 anti-SHIP Rabbit Monoclonal antibody reactive to the isotypes of INPP5D?
C. Krishna
Verified customer
Asked: 2017-04-27
Answer
The immunogen of M03358 anti-SHIP Rabbit Monoclonal antibody is A synthesized peptide derived from human SHIP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-27
Question
My question regarding product M03358, anti-SHIP Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Thomas
Verified customer
Asked: 2014-12-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03358 anti-SHIP Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-12-23