Data

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Key=store_id
Key=entity_id, value=2298
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=EK0793
Key=has_options, value=0
Key=required_options, value=0
Key=created_at, value=2014-09-17 11:44:10
Key=updated_at, value=2024-09-26 00:45:59
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Human IL-17E/IL-25 ELISA Kit PicoKine®
Key=cj_search_index
Key=publication_count, value=1
Key=qty_in_warehouses
Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
Key=tax_class_id, value=Taxable Goods
Key=featured, value=No
Key=featured_image, value=Yes
Key=fb_product, value=
Key=custom_product_page, value=No
Key=free_shipping, value=No
Key=template, value=elisa-kits
Key=quantity_and_stock_status
Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1365
Key=yoast_content_score, value=60
Key=price, value=499.0000
Key=weight, value=1.0000
Key=meta_title, value=Human IL-17E/IL-25 ELISA Kit PicoKine® EK0793 | Boster Bio
Key=meta_description, value=Human IL-17E/IL-25 ELISA Kit PicoKine® EK0793 is a quantitative ELISA for measuring IL25 in Human cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Order now!
Key=image, value=/e/k/ek0793_1.png
Key=small_image, value=/e/k/ek0793_1.png
Key=thumbnail, value=/e/k/ek0793_1.png
Key=url_key, value=human-il-17e-il-25-picokine-trade-elisa-kit-ek0793-boster
Key=url_path, value=human-il-17e-il-25-picokine-trade-elisa-kit-ek0793-boster
Key=options_container, value=Product Info Column
Key=image_label, value=Human IL-17E/IL-25 PicoKine ELISA Kit standard curve
Key=small_image_label, value=Human IL-17E/IL-25 PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Human IL-17E/IL-25 PicoKine ELISA Kit standard curve
Key=msrp_display_actual_price_type, value=
Key=gift_message_available, value=No
Key=size, value=96 wells/kit, with removable strips.
Key=protein_name, value=Interleukin-25
Key=gene_full_name, value=Interleukin-25
Key=uniprot_id, value=Q9H293
Key=assay_range, value=62.5 pg/ml - 4,000 pg/ml
Key=sensitivity, value=<10 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=principle, value=monoclonal antibody from mouse|polyclonal antibody from goat
Key=anticoagulant, value=heparin or EDTA
Key=assay_procedure_step_1, value=4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml
Key=tmb_incubation_time, value=15-25min
Key=predicted_reactivity
Key=source_company, value=Boster EK0793
Key=molecular_weight, value=20330 MW
Key=related_product_skus, value=PROTQ9H293,PROTQ8VHH8
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Cytokines, Immunology, Innate Immunity, Interleukins
Key=rating_value, value=100
Key=review_count, value=4
Key=phospho_site
Key=mp_exclude_sitemap, value=Yes
Key=cj_related_products, value=Airway Disease:PA1782-1|A00225-2|EKC2186|A00225-3^Helminthiasis:A09407-1
Key=description, value=Human IL-17E/IL-25 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Human IL-17E/IL-25 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=meta_keyword, value=Human IL-17E/IL-25 ELISA Kit PicoKine, Bosterbio High Sensitivity
Key=background, value=Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-κB activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.
Key=gene_name, value=IL25
Key=synonyms, value=Interleukin-25;IL-25;Interleukin-17E;IL-17E;IL25;IL17E;UNQ3120/PRO10272;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=10 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	162	689	1026	168	748	1001
Standard deviation	8.91	42.02	50.27	10.92	57.59	53.05
CV (%)	5.5%	6.1%	4.9%	6.5%	7.7%	5.3%

Key=application_details, value=For serum, dilution ratio is 1:1 and concentration is less than the lowest standard, 62.5 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 90 pg/ml.
Key=subcellular_localization, value= Secreted.
Key=sequence_similarities, value=Belongs to the IL-17 family.
Key=tissue_specificity, value= Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
Key=protein_function, value= Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
Key=sample_data, value=

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.012	0.070	0.125	0.235	0.420	0.840	1.611	2.011

Key=competitor_equivalent_skus, value=
Key=immunogen, value=Expression system for standard: E.coli; Immunogen sequence: Y33-G177
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	162	151	150	157	155	4.84	3.1%
Sample 2	689	601	681	631	650	36.2	5.5%
Sample 3	1026	1036	1126	1127	1078	47.88	4.4%

*number of samples for each test n=16.
Key=options
Key=media_gallery
Key=extension_attributes
Key=tier_price
Key=tier_price_changed
Key=category_ids
Key=is_salable
Key=website_ids
Key=request_path
Key=rating_summary
Key=_cache_instance_store_filter
Key=media_gallery_images