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Product Info Summary
| SKU: | EK0793 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IL-17E/IL-25 ELISA Kit PicoKine®
View all IL-17E/IL-25 ELISA kits
SKU/Catalog Number
EK0793
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-17E/IL-25 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-17E/IL-25 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0793)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: Y33-G177
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0793 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0793 is reactive to IL25 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0793 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-17E/IL-25
Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-κB activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0793-CAP | Anti-Human IL25 Pre-coated 96-well strip microplate | 1 |
| EK0793-ST | Human IL25 Standard | 2 vials, 10 ng/tube |
| EK0793-DA | Human IL25 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.012 | 0.070 | 0.125 | 0.235 | 0.420 | 0.840 | 1.611 | 2.011 |
Data Example Images
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Human IL-17E/IL-25 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-17E/IL-25, For serum, dilution ratio is 1:1 and concentration is less than the lowest standard, 62.5 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 90 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-17E/IL-25 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 162 | 689 | 1026 | 168 | 748 | 1001 |
| Standard deviation | 8.91 | 42.02 | 50.27 | 10.92 | 57.59 | 53.05 |
| CV (%) | 5.5% | 6.1% | 4.9% | 6.5% | 7.7% | 5.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-17E/IL-25 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 162 | 151 | 150 | 157 | 155 | 4.84 | 3.1% |
| Sample 2 | 689 | 601 | 681 | 631 | 650 | 36.2 | 5.5% |
| Sample 3 | 1026 | 1036 | 1126 | 1127 | 1078 | 47.88 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For IL25 (Source: Uniprot.Org, NCBI)
Gene Name
IL25
Full Name
Interleukin-25
Weight
20330 MW
Superfamily
IL-17 family
Alternative Names
IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25 IL25 IL17E interleukin 25 interleukin-25|interleukin-17E
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL25, check out the IL25 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL25: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-17E/IL-25 ELISA Kit PicoKine® (EK0793)
Hello CJ!
EK0793 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Relationship of Microbial Profile With Airway Immune Response in Eosinophilic or Neutrophilic Inflammation of Asthmatics
Upregulation of interleukin-33 and thymic stromal lymphopoietin levels in the lungs of idiopathic pulmonary fibrosis
The role of IL-25 and IL-33 in chronic rhinosinusitis with or without nasal polyps
Meng H, Shen Y, Shen J, Zhou F, Shen S, Das Un. Lipids Health Dis. 2013 Oct 29;12:160. Doi: 10.1186/1476-511X-12-160. Effect Of N-3 And N-6 Unsaturated Fatty Acids On Prostate Cancer (Pc-3) And Prostate Epithelial (Rwpe-1) Cells In Vitro.
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Human IL-17E/IL-25 ELISA Kit PicoKine®
Question
What do you suggest using for controls/calibrators for EK0793?
Verified customer
Asked: 2021-01-26
Answer
Unfortunately, we could only provide recombinant proteins as positive controls which are compatible only with our ELISA kits. For controls such as serum, plasma, we suggest reviewing relevant publications for how to prepare them.
Boster Scientific Support
Answered: 2021-01-27
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
S. Clark
Verified customer
Asked: 2020-06-11
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-06-11
Question
One of our customer bought Human IL-17E/IL-25 PicoKine
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
COA is sent.
Boster Scientific Support
Answered: 2020-03-24
Question
Can EK0793 be used with mouse samples?
Verified customer
Asked: 2019-10-18
Answer
Our lab doesn't recommend working on mouse samples using the Human IL-17E/IL-25 ELISA Kit PicoKine™ (EK0793).
Boster Scientific Support
Answered: 2019-10-21
Question
Can EK0789 and EK0793 be used with skin biopsies? If yes, is there a need to modify the protocol?
Verified customer
Asked: 2019-03-27
Answer
Our lab has not worked on skin biopsies using the Human IL-17C ELISA Kit PicoKine™ (EK0789) and Human IL-17E/IL-25 ELISA Kit PicoKine™ (EK0793). We suggest you to run a pilot test using the kits. The following is the suggested sample preparation protocol; 1. Mince skin biopsies and then rinse skin biopsies in ice-cold PBS. 2. Place minced skin biopsies in tissue homogenizer. 3. Add ice-cold PBS. For a 0.5g piece of tissue, add 5ml of ice-cold PBS (Volumes of PBS must be determined in relation to the amount of skin biopsies present.) 5. Homogenize for several minutes at high speed. 6. Sonicate until no skin biopsies chunks remain. 7. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the sample type. 8. Aspirate supernatant. 9. Aliquot supernatant and store at -20°C. There is no need to adjust the main protocol.
Boster Scientific Support
Answered: 2019-03-28
Question
Can EK0793 be used with skin biopsies? If yes, is there a need to modify the protocol?
Verified customer
Asked: 2019-03-27
Answer
Our lab has not worked on skin biopsies using Human IL-17E/IL-25 ELISA Kit PicoKine™ (EK0793). We suggest you to run a pilot test using the kits. The following is the suggested sample preparation protocol. 1. Mince skin biopsies and then rinse skin biopsies in ice-cold PBS. 2. Place minced skin biopsies in tissue homogenizer. 3. Add ice-cold PBS. For a 0.5g piece of tissue, add 5ml of ice-cold PBS (Volumes of PBS must be determined in relation to the amount of skin biopsies present.) 5. Homogenize for several minutes at high speed. 6. Sonicate until no skin biopsies chunks remain. 7. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the sample type. 8. Aspirate supernatant. 9. Aliquot supernatant and store at -20°C. There is no need to adjust the main protocol.
Boster Scientific Support
Answered: 2019-03-28
Question
Q: Can IL-17E ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-03-09
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-03-09
Question
Q: how to proceed with the analysis of ELISA data? I have obtained IL-17E level in cell lysates.
Verified Customer
Verified customer
Asked: 2019-01-20
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-01-20
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-02-09
Question
Q: if the enzyme conjugated IL-17E antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-17E antigen?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-09
Question
Q: how to thaw whole blood sample for IL-17E ELISA after freezing?
J. Varadkar
Verified customer
Asked: 2016-04-09
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17E for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-04-09
Question
Q: What is the optimal O.D. value for IL-17E ELISA kit? I used your IL-17E ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-17E even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2015-11-08
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-17E ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-17E that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2015-11-08
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-17E using the EK0793 Human IL-17E Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-09-24
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2015-09-24
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
R. Young
Verified customer
Asked: 2014-07-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-07-06
