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Product Info Summary
| SKU: | A03358 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SHIP/INPP5D Antibody Picoband®
SKU/Catalog Number
A03358
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SHIP/INPP5D Antibody Picoband® catalog # A03358. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHIP/INPP5D Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03358)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SHIP, which shares 95.1% and 97.6% amino acid (aa) sequence identity with mouse and rat SHIP, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03358 is reactive to INPP5D in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
145 kDa
Calculated molecular weight
133.292kDa
Background of SHIP
Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 is an enzyme that in humans is encoded by the INPP5D gene. This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03358 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section),0.5-1μg/ml
Positive Control
WB: human CCRF-CEM whole cell, human SW620 whole cell
IHC: human tonsil tissue, mouse spleen tissue, rat spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SHIP using anti-SHIP antibody (A03358).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human CCRF-CEM whole cell lysates,
Lane 2: human SW620 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHIP antigen affinity purified polyclonal antibody (Catalog # A03358) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHIP at approximately 145KD. The expected band size for SHIP is at 133KD.
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Figure 2. IHC analysis of SHIP using anti-SHIP antibody (A03358).
SHIP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SHIP Antibody (A03358) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of SHIP using anti-SHIP antibody (A03358).
SHIP was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SHIP Antibody (A03358) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SHIP using anti-SHIP antibody (A03358).
SHIP was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SHIP Antibody (A03358) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For INPP5D (Source: Uniprot.org, NCBI)
Gene Name
INPP5D
Full Name
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Weight
133.292kDa
Superfamily
inositol 1,4,5-trisphosphate 5-phosphatase family
Alternative Names
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; Inositol polyphosphate-5-phosphatase of 145 kDa; SIP-145; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SHIP-1; p150Ship; hp51CN; INPP5D; SHIP; SHIP1 INPP5D SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship inositol polyphosphate-5-phosphatase D phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1|SH2 domain-containing inositol 5-phosphatase 1|inositol polyphosphate-5-phosphatase, 145kD|inositol polyphosphate-5-phosphatase, 145kDa|phosphatidylinositol 4,5-bisphosphate 5-phosphatase|signaling inositol polyphosphate 5 phosphatase SIP-145|signaling inositol polyphosphate phosphatase SHIP II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INPP5D, check out the INPP5D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INPP5D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHIP/INPP5D Antibody Picoband® (A03358)
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No publications found for A03358
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Customer Q&As
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1 Customer Q&As for Anti-SHIP/INPP5D Antibody Picoband®
Question
We are currently using anti-SHIP/INPP5D antibody A03358 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
A. Wu
Verified customer
Asked: 2016-07-14
Answer
The anti-SHIP/INPP5D antibody (A03358) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-14
