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Product Info Summary
| SKU: | A08290 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Setdb2 Antibody
SKU/Catalog Number
A08290
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Setdb2 Antibody (Catalog # A08290). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Setdb2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08290)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Setdb2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length protein corresponding to mouse Setdb2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08290 is reactive to SETDB2 in Human, Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
33342 MW
Background of SETDB2
Setdb2 and the neighboring gene Phf11 were linked to familial atopy/ asthma and are in a "critical region" commonly deleted in CLL. Setdb2 is a candidate histone methyltransferase as a member of the Su(var) 3-9 family of SET domain-containing enzymes. Other predicted domains in Setdb2 include a methylcytosine binding domain and a large bifurcation domain interrupting the putative histone methyltransferase (SET) domain whose function is unknown. Anti-Setdb2 antibody is ideal for researchers interested in Cancer Research and Epigenetics Research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08290 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,500 - 1:10,000
WB: 1:10,000 - 1:100,000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Setdb2 using anti-Setdb2 antibody (A08290).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Setdb2 antigen affinity purified polyclonal antibody (Catalog # A08290) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Setdb2.
Protein Target Info & Infographic
Gene/Protein Information For SETDB2 (Source: Uniprot.org, NCBI)
Gene Name
SETDB2
Full Name
Histone-lysine N-methyltransferase SETDB2
Weight
33342 MW
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
Setdb2, Histone-lysine N-methyltransferase SETDB2, SET domain bifurcated 2, Setdb 2, Gm293 SETDB2 C13orf4, CLLD8, CLLL8, KMT1F SET domain bifurcated histone lysine methyltransferase 2 histone-lysine N-methyltransferase SETDB2|SET domain bifurcated 2|chronic lymphocytic leukemia deletion region gene 8 protein|lysine N-methyltransferase 1F
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SETDB2, check out the SETDB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SETDB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Setdb2 Antibody (A08290)
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No publications found for A08290
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Customer Q&As
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5 Customer Q&As for Anti-Setdb2 Antibody
Question
We are currently using anti-Setdb2 antibody A08290 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The anti-Setdb2 antibody (A08290) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-11
Question
Is a blocking peptide available for product anti-Setdb2 antibody (A08290)?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We do provide the blocking peptide for product anti-Setdb2 antibody (A08290). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-12
Question
I was wanting to use your anti-Setdb2 antibody for WB for human colon testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon testis identification?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
As indicated on the product datasheet, A08290 anti-Setdb2 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human colon testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-29
Question
Would A08290 anti-Setdb2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
You can see on the product datasheet, A08290 anti-Setdb2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-07
Question
Here is the WB image, lot number and protocol we used for colon testis using anti-Setdb2 antibody A08290. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-25
