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Product Info Summary
| SKU: | A08290-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CLLD8/SETDB2 Picoband® Antibody
SKU/Catalog Number
A08290-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CLLD8/SETDB2 Picoband® Antibody catalog # A08290-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLLD8/SETDB2 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08290-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CLLD8/SETDB2, which shares 96.6% and 93.1% amino acid (aa) sequence identity with mouse and rat CLLD8/SETDB2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08290-2 is reactive to SETDB2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
82 kDa
Calculated molecular weight
81.894kDa
Background of SETDB2
SET domain bifurcated histone lysine methyltransferase 2 is a protein in humans encoded by the SETDB2 gene. It is mapped to 13q14.2. This gene encodes a member of a family of proteins that contain a methyl-CpG-binding domain (MBD) and a SET domain and function as histone methyltransferases. This protein is recruited to heterochromatin and plays a role in the regulation of chromosome segregation. This region is commonly deleted in chronic lymphocytic leukemia. Naturally-occuring readthrough transcription occurs from this gene to the downstream PHF11 (PHD finger protein 11) gene. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08290-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human THP-1 whole cell, rat liver tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SETDB2 using anti-SETDB2 antibody (A08290-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETDB2 antigen affinity purified polyclonal antibody (Catalog # A08290-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SETDB2 at approximately 82KD. The expected band size for SETDB2 is at 82KD.
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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-SETDB2 antibody (A07692-1).
Overlay histogram showing THP-1 cells stained with A07692-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SETDB2 Antibody (A07692-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SETDB2 (Source: Uniprot.org, NCBI)
Gene Name
SETDB2
Full Name
Histone-lysine N-methyltransferase SETDB2
Weight
81.894kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
Histone-lysine N-methyltransferase SETDB2; Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2; SETDB2; C13orf4; CLLD8; KMT1F SETDB2 C13orf4, CLLD8, CLLL8, KMT1F SET domain bifurcated histone lysine methyltransferase 2 histone-lysine N-methyltransferase SETDB2|SET domain bifurcated 2|chronic lymphocytic leukemia deletion region gene 8 protein|lysine N-methyltransferase 1F
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SETDB2, check out the SETDB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SETDB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLLD8/SETDB2 Picoband® Antibody (A08290-2)
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