Product Info Summary
SKU: | A05624-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SLC9A3R2/Nherf 2 Antibody
SKU/Catalog Number
A05624-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SLC9A3R2/Nherf 2 Antibody (Catalog # A05624-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLC9A3R2/Nherf 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05624-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SLC9A3R2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05624-1 is reactive to SLC9A3R2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
37414 MW
Background of NHERF-2
Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.
Poulat F., J. Biol. Chem. 272:7167-7172(1997) [PubMed: 9054412].
Reczek D.,J. Cell Biol. 139:169-179(1997) [PubMed: 9314537].
Yun C.H.C., Proc. Natl. Acad. Sci. U.S.A. 94:3010-3015(1997) [PubMed: 9096337].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05624-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from JK cells and K562 cells, using SLC9A3R2 antibody A05624-1.
Protein Target Info & Infographic
Gene/Protein Information For SLC9A3R2 (Source: Uniprot.org, NCBI)
Gene Name
SLC9A3R2
Full Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Weight
37414 MW
Alternative Names
Na (+)/H (+) exchange regulatory cofactor NHE-RF2;NHERF-2;NHE3 kinase A regulatory protein E3KARP;SRY-interacting protein 1;SIP-1;Sodium-hydrogen exchanger regulatory factor 2;Solute carrier family 9 isoform A3 regulatory factor 2;Tyrosine kinase activator protein 1;TKA-1;SLC9A3R2;NHERF2; SLC9A3R2 E3KARP, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1 SLC9A3 regulator 2 Na(+)/H(+) exchange regulatory cofactor NHE-RF2|NHE3 kinase A regulatory protein E3KARP|NHE3 regulatory factor 2|SRY-interacting protein 1|sodium/hydrogen exchanger 3 kinase A regulatory protein|solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2|solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2|tyrosine kinase activator protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC9A3R2, check out the SLC9A3R2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC9A3R2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC9A3R2/Nherf 2 Antibody (A05624-1)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-SLC9A3R2/Nherf 2 Antibody
Question
Can you help my question with product A05624-1, anti-SLC9A3R2/Nherf 2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05624-1 anti-SLC9A3R2/Nherf 2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-SLC9A3R2/Nherf 2 antibody A05624-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
The anti-SLC9A3R2/Nherf 2 antibody (A05624-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-17
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-SLC9A3R2/Nherf 2 antibody A05624-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-03-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-28
Question
Would anti-SLC9A3R2/Nherf 2 antibody A05624-1 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
According to the expression profile of cervix carcinoma erythroleukemia, SLC9A3R2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-SLC9A3R2/Nherf 2 antibody A05624-1 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2017-09-13
Question
Is this A05624-1 anti-SLC9A3R2/Nherf 2 antibody reactive to the isotypes of SLC9A3R2?
J. Lewis
Verified customer
Asked: 2016-09-06
Answer
The immunogen of A05624-1 anti-SLC9A3R2/Nherf 2 antibody is Synthesized peptide derived from internal of human SLC9A3R2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-09-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-SLC9A3R2/Nherf 2 antibody A05624-1. Let me know if you need anything else.
L. Brown
Verified customer
Asked: 2013-07-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-03