Product Info Summary
SKU: | A09411-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SEPT4/SEPTIN4 Antibody Picoband®
SKU/Catalog Number
A09411-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SEPT4/SEPTIN4 Antibody Picoband® catalog # A09411-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SEPT4/SEPTIN4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09411-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SEPT4/SEPTIN4 recombinant protein (Position: E18-K451).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09411-2 is reactive to SEPTIN4 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
55.098kDa
Background of SEPTIN4
Septin 4 is a protein that in humans is encoded by the gene SEPTIN4. This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09411-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: rat brain tissue, mouse brain tissue
FCM: HEL cell, Hela cell
Validation Images & Assay Conditions
![a09411 2 septin4 primary antibodies wb testing 1 a09411 2 septin4 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a09411-2-septin4-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of SEPT4/SEPTIN4 using anti-SEPT4/SEPTIN4 antibody (A09411-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT4/SEPTIN4 antigen affinity purified polyclonal antibody (Catalog # A09411-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPT4/SEPTIN4 at approximately 50 kDa. The expected band size for SEPT4/SEPTIN4 is at 50 kDa.
![a09411 2 septin4 primary antibodies fcm testing 2 a09411 2 septin4 primary antibodies fcm testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a09411-2-septin4-primary-antibodies-fcm-testing-2.jpg)
Click image to see more details
Figure 2. Flow Cytometry analysis of HEL cells using anti-SEPT4/SEPTIN4 antibody (A09411-2).
Overlay histogram showing HEL cells stained with A09411-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT4/SEPTIN4 Antibody (A09411-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a09411 2 septin4 primary antibodies fcm testing 3_1 a09411 2 septin4 primary antibodies fcm testing 3_1](https://www.bosterbio.com/media/catalog/product/a/0/a09411-2-septin4-primary-antibodies-fcm-testing-3_1.jpg)
Click image to see more details
Figure 3. Flow Cytometry analysis of Hela cells using anti-SEPT4/SEPTIN4 antibody (A09411-2).
Overlay histogram showing Hela cells stained with A09411-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT4/SEPTIN4 Antibody (A09411-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SEPTIN4 (Source: Uniprot.org, NCBI)
Gene Name
SEPTIN4
Full Name
Septin-4
Weight
55.098kDa
Superfamily
TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily
Alternative Names
Mediator of RNA polymerase II transcription subunit 20; Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP; MED20; TRFP; SEPTIN4 ARTS, BRADEION, C17orf47, CE5B3, H5, MART, PNUTL2, SEP4, SEPT4, hCDCREL-2, hucep-7 septin 4 septin-4|CE5B3 beta|apoptosis-related protein in the TGF-beta signaling pathway|bradeion beta|brain protein H5|cell division control-related protein 2|cerebral protein 7|peanut-like protein 2|septin-M|uncharacterized protein C17orf47
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEPTIN4, check out the SEPTIN4 Infographic
![SEPTIN4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEPTIN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SEPT4/SEPTIN4 Antibody Picoband® (A09411-2)
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