Anti-SCUBE3 Antibody

Product Name

Anti-SCUBE3 Antibody

View all SCUBE3 Antibodies

SKU/Catalog Number

A09090

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SCUBE3 Antibody (Catalog # A09090). Tested in ELISA, IHC-P applications. This antibody reacts with Human.

Storage & Handling

SCUBE3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-SCUBE3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09090)

Host

Rabbit

Contents

SCUBE3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SCUBE3 antibody was raised against a 15 amino acid peptide near the center of human SCUBE3. The immunogen is located within amino acids 440 - 490 of SCUBE3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ATAD3B antibody is predicted to not cross-react with other ATAD protein family members. At least three isoforms of ATAD3B are known to exist.

Reactive Species

A09090 is reactive to SCUBE3 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

109282 MW

Background of SCUBE3

SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart (1,2). Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth. SCUBE3 has been shown to be an endogenous TGF-beta receptor ligand (3,4) and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-beta1, VEGF, Snail, and Slug.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09090 is guaranteed for ELISA, IHC-P Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SCUBE3 antibody can be used for detection of SCUBE3 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For SCUBE3 (Source: Uniprot.org, NCBI)

Alternative Names

CEGF3, CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3 SCUBE3 CEGF3, SSFSC2 signal peptide, CUB domain and EGF like domain containing 3 signal peptide, CUB and EGF-like domain-containing protein 3|CUB domain and EGF-like repeat containing 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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