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Product Info Summary
| SKU: | A09090-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-SCUBE3 Antibody
SKU/Catalog Number
A09090-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SCUBE3 Antibody (Catalog # A09090-1). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SCUBE3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SCUBE3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09090-1)
Host
Rabbit
Contents
SCUBE3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SCUBE3 antibody was raised against a 15 amino acid synthetic peptide near the center of human SCUBE3. The immunogen is located within amino acids 210 - 260 of SCUBE3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of SCUBE3 are known to exist. SCUBE3 antibody is predicted to not cross-react with other SCUBE members
Reactive Species
A09090-1 is reactive to SCUBE3 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
109282 MW
Background of SCUBE3
SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart. Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth.. SCUBE3 has been shown to be an endogenous TGF-β receptor ligand and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-β receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-β1, VEGF, Snail, and Slug.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09090-1 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SCUBE3 antibody can be used for detection of SCUBE3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SCUBE3 in mouse kidney tissue lysate with SCUBE3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of SCUBE3 in human kidney tissue with SCUBE3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SCUBE3 (Source: Uniprot.org, NCBI)
Gene Name
SCUBE3
Full Name
Signal peptide, CUB and EGF-like domain-containing protein 3
Weight
109282 MW
Alternative Names
CEGF3, CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3 SCUBE3 CEGF3, SSFSC2 signal peptide, CUB domain and EGF like domain containing 3 signal peptide, CUB and EGF-like domain-containing protein 3|CUB domain and EGF-like repeat containing 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCUBE3, check out the SCUBE3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCUBE3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCUBE3 Antibody (A09090-1)
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