Human M-CSF ELISA Quick Kit PicoKine®

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human M-CSF ELISA Quick Kit PicoKine®

See all M-CSF products

SKU/Catalog Number

FEK0444

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human M-CSF ELISA Kit PicoKine® (120 minutes, 96 Tests). Quantitate Human CSF1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human M-CSF ELISA Quick Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0444)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: E33-S190

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0444 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0444 is reactive to CSF1 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Application Guarantee

FEK0444 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of M-CSF

M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.

Kit Components

Catalog number	Description	Quantity
FEK0444-CAP	96-well plate precoated with anti-Human M-CSF antibody	1
EK0444-ST	lyophilized recombinant Human M-CSF standard	10 ng/tube
FEK0444-DA	Biotinylated anti-Human M-CSF detection antibody (50x)	100 ul
-	Avidin-Biotin-Peroxidase Complex (30x)	400 µl
AR1106-1	Sample diluent	30 ml
AR1106-2	Antibody diluent	12 ml
-	Avidin-Biotin-Peroxidase Diluent	12 ml
AR1106-5	Wash Buffer (25x)	20 ml
AR1104	Color Developing Reagent (TMB)	10 ml
AR1105	Stop Solution	10 ml
PLA-SEA	Plate Sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.004	0.128	0.299	0.537	0.992	1.347	1.803	2.095

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human M-CSF ELISA Quick Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of M-CSF in ELISA kits from four different production batches/lots.

Gene/Protein Information For CSF1 (Source: Uniprot.Org, NCBI)

Alternative Names

Macrophage colony-stimulating factor 1;CSF-1;M-CSF;MCSF;Lanimostim;Processed macrophage colony-stimulating factor 1;CSF1; CSF1 CSF-1, MCSF colony stimulating factor 1 macrophage colony-stimulating factor 1|colony stimulating factor 1 (macrophage)|lanimostim|macrophage colony stimulating factor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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