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Product Info Summary
| SKU: | A30653 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-RPC5 POLR3E Antibody
SKU/Catalog Number
A30653
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RPC5 POLR3E Antibody (Catalog# A30653). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPC5 POLR3E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30653)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RPC5. Immunogen sequence location: 191-240
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30653 is reactive to POLR3E in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
79898 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30653 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RPC5 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of LOVO cells, using RPC5 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For POLR3E (Source: Uniprot.org, NCBI)
Gene Name
POLR3E
Full Name
DNA-directed RNA polymerase III subunit RPC5
Weight
79898 MW
Alternative Names
POLR3E;KIAA1452;DNA-directed RNA polymerase III subunit RPC5;RNA polymerase III subunit C5;DNA-directed RNA polymerase III 80 kDa polypeptide Polr3e|RP, RPC5, Sin|polymerase (RNA) III (DNA directed) polypeptide E|DNA-directed RNA polymerase III subunit RPC5|RNA polymerase III polypeptide E|RNA polymerase III subunit 5|RNA polymerase III subunit C5|sex-lethal interactor homolog|sxl interactor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR3E, check out the POLR3E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR3E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPC5 POLR3E Antibody (A30653)
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No publications found for A30653
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Customer Q&As
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1 Customer Q&As for Anti-RPC5 POLR3E Antibody
Question
We are currently using anti-RPC5 antibody A30653 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
The anti-RPC5 antibody (A30653) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-25
