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Product Info Summary
| SKU: | EK2119 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine®
View all Crossveinless-2/CV-2/BMPER ELISA kits
SKU/Catalog Number
EK2119
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Bmper in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2119)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: V34-R685
Sensitivity
<50 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2119 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2119 is reactive to Bmper in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2119 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Crossveinless-2/CV-2/BMPER
This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2119-CAP | Anti-Mouse Bmper Pre-coated 96-well strip microplate | 1 |
| EK2119-ST | Mouse Bmper Standard | 2 vials, 10 ng/tube |
| EK2119-DA | Mouse Bmper Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.059 | 0.143 | 0.193 | 0.307 | 0.562 | 0.917 | 1.452 | 2.098 |
Data Example Images
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Mouse BMPER PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Crossveinless-2/CV-2/BMPER, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 62.5 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 207 | 813 | 1459 | 212 | 846 | 1558 |
| Standard deviation | 11.18 | 46.34 | 97.75 | 12.30 | 62.60 | 123.08 |
| CV (%) | 5.4% | 5.7% | 6.7% | 5.8% | 7.4% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Crossveinless-2/CV-2/BMPER in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 207 | 220 | 215 | 195 | 209 | 7.94 | 3.8% |
| Sample 2 | 813 | 827 | 854 | 792 | 822 | 46.85 | 5.7% |
| Sample 3 | 1459 | 1322 | 1478 | 1334 | 1398 | 86.68 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For Bmper (Source: Uniprot.Org, NCBI)
Gene Name
Bmper
Full Name
BMP-binding endothelial regulator protein
Weight
76.148kDa
Alternative Names
BMP binding endothelial regulator; BMP-binding endothelial regulator precursor protein; BMP-binding endothelial regulator protein; BMPER; Bone morphogenetic protein-binding endothelial cell precursor-derived regulator; CRIM3; crossveinless 2; Crossveinless-2; CV2; CV-2; hCV2; KIAA1965; Protein crossveinless-2 Bmper|3110056H04Rik, CV-, CV-2, Cri, Crim3, Cv, Cv2|BMP-binding endothelial regulator|BMP-binding endothelial regulator protein|bone morphogenetic protein-binding endothelial cell precursor-derived regulator|crossveinless-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Bmper, check out the Bmper Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Bmper: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® (EK2119)
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No publications found for EK2119
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine®
Question
Does EK2119 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® (EK2119) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: how to proceed with the analysis of ELISA data? I measured BMPER level in serum.
Verified Customer
Verified customer
Asked: 2020-09-03
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-09-03
Question
Q: how to thaw whole blood sample for BMPER ELISA after freezing?
B. White
Verified customer
Asked: 2020-01-04
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMPER for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-01-04
Question
Q: can you recommend the dilution ratio of serum samples for detection of BMPER in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the BMPER ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-10-14
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
J. Carter
Verified customer
Asked: 2019-06-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-06-10
Question
Q: Can BMPER ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
N. Anand
Verified customer
Asked: 2016-08-26
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-08-26
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
W. Zhang
Verified customer
Asked: 2014-10-08
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-10-08
