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Product Info Summary
| SKU: | A11932 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-POLR3E Antibody
SKU/Catalog Number
A11932
Size
100μl
Form
Liquid
Description
Boster Bio Anti-POLR3E Antibody catalog # A11932. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLR3E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11932)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RPC5. AA range:191-240
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11932 is reactive to POLR3E in Human, Mouse
Reconstitution
Calculated molecular weight
79898 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11932 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of JK cells using POLR3E Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using POLR3E Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For POLR3E (Source: Uniprot.org, NCBI)
Gene Name
POLR3E
Full Name
DNA-directed RNA polymerase III subunit RPC5
Weight
79898 MW
Alternative Names
DNA-directed RNA polymerase III subunit RPC5;RNA polymerase III subunit C5;DNA-directed RNA polymerase III 80 kDa polypeptide;POLR3E;KIAA1452; Polr3e|RP, RPC5, Sin|polymerase (RNA) III (DNA directed) polypeptide E|DNA-directed RNA polymerase III subunit RPC5|RNA polymerase III polypeptide E|RNA polymerase III subunit 5|RNA polymerase III subunit C5|sex-lethal interactor homolog|sxl interactor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR3E, check out the POLR3E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR3E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLR3E Antibody (A11932)
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No publications found for A11932
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-POLR3E Antibody
Question
Do you have a BSA free version of anti-POLR3E antibody A11932 available?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-POLR3E antibody A11932 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-03
Question
My question regarding product A11932, anti-POLR3E antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
W. Li
Verified customer
Asked: 2019-11-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11932 anti-POLR3E antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-29
Question
I see that the anti-POLR3E antibody A11932 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-POLR3E antibody A11932. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-27
Question
I was wanting to use your anti-POLR3E antibody for WB for mouse embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryonic kidney identification?
M. Bhatt
Verified customer
Asked: 2017-10-04
Answer
As indicated on the product datasheet, A11932 anti-POLR3E antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-04
Question
See attached the WB image, lot number and protocol we used for embryonic kidney using anti-POLR3E antibody A11932. Please let me know if you require anything else.
E. Miller
Verified customer
Asked: 2014-03-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-27
