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Product Info Summary
| SKU: | PB9116 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RIP/RIPK1 Antibody Picoband®
View all RIPK1/RIP1 Antibodies
SKU/Catalog Number
PB9116
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RIP/RIPK1 Antibody Picoband® catalog # PB9116. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RIP/RIPK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9116)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RIP recombinant protein (Position: K316-N671). Human RIP shares 65% amino acid (aa) sequence identity with mouse RIP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9116 is reactive to RIPK1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
76 kDa
Calculated molecular weight
75931 MW
Background of RIPK1/RIP1
RIPK1, also known as RIP or RIP1, is an enzyme that in humans is encoded by the RIPK1 gene. It is mapped to 6p25.2. RIPK1 is a key signaling molecule in the programmed necrosis pathway, which plays important roles in development, tissue damage response, and antiviral immunity. RIPK1 is known to have function in a variety of cellular pathways including the NF-κB pathway and programmed necrotic cell death (necroptosis). The kinase domain, while important for necroptotic (programmed necrotic) functions, it appears dispensable for other lethal, as well as pro-survival roles. Also, proteolytic processing of RIPk1, through both caspase-dependent and -independent mechanisms, triggers lethality that is dependent on the generation of one or more specific C-terminal cleavage product (s) of RIPk1 upon stress.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9116 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Jurkat whole cell, human 22RV1 whole cell, human MCF-7 whole cell, human Hela whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RIP using anti-RIP antibody (PB9116).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human RIP protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP antigen affinity purified polyclonal antibody (Catalog # PB9116) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP at approximately 38 kDa. The expected band size for RIP is at 38 kDa.
Click image to see more details
Figure 2. Western blot analysis of RIP using anti-RIP antibody (PB9116).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human 22RV1 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP antigen affinity purified polyclonal antibody (Catalog # PB9116) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP at approximately 76 kDa. The expected band size for RIP is at 76 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RIPK1 (Source: Uniprot.org, NCBI)
Gene Name
RIPK1
Full Name
Receptor-interacting serine/threonine-protein kinase 1
Weight
75931 MW
Superfamily
protein kinase superfamily
Alternative Names
Receptor-interacting serine/threonine-protein kinase 1;2.7.11.1;Cell death protein RIP;Receptor-interacting protein 1;RIP-1;Serine/threonine-protein kinase RIP;RIPK1;RIP, RIP1; RIPK1 AIEFL, IMD57, RIP, RIP-1, RIP1 receptor interacting serine/threonine kinase 1 receptor-interacting serine/threonine-protein kinase 1|cell death protein RIP|receptor (TNFRSF)-interacting serine-threonine kinase 1|receptor-interacting protein 1|receptor-interacting protein kinase 1|serine/threonine-protein kinase RIP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIPK1, check out the RIPK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RIP/RIPK1 Antibody Picoband® (PB9116)
Hello CJ!
PB9116 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Smad3-Targeted Therapy Protects against Cisplatin-Induced AKI by Attenuating Programmed Cell Death and Inflammation via a NOX4-Dependent Mechanism. Qin Yang,Li Gao,Xiao-wei Hu,Jia-nan Wang,Yao Zhang,Yu-hang Dong,Hui Yao Lan,Xiao-ming Meng
Species: Mouse
PB9116 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:NA
Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors
Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.
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Customer Q&As
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15 Customer Q&As for Anti-RIP/RIPK1 Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical vein endothelial cell using anti-RIP/RIPK1 antibody PB9116. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-23
Question
Please see the WB image, lot number and protocol we used for umbilical vein endothelial cell using anti-RIP/RIPK1 antibody PB9116. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-20
Question
Is this PB9116 anti-RIP/RIPK1 antibody reactive to the isotypes of RIPK1?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The immunogen of PB9116 anti-RIP/RIPK1 antibody is E.coli-derived human RIP recombinant protein (Position: K316-N671). Human RIP shares 65% amino acid (aa) sequence identity with mouse RIP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-13
Question
Does anti-RIP/RIPK1 antibody PB9116 work for WB with umbilical vein endothelial cell?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
According to the expression profile of umbilical vein endothelial cell, RIPK1 is highly expressed in umbilical vein endothelial cell. So, it is likely that anti-RIP/RIPK1 antibody PB9116 will work for WB with umbilical vein endothelial cell.
Boster Scientific Support
Answered: 2019-09-25
Question
Our team were satisfied with the WB result of your anti-RIP/RIPK1 antibody. However we have seen positive staining in right lobe of liver cytoplasm. cell membrane. using this antibody. Is that expected? Could you tell me where is RIPK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
Based on literature, right lobe of liver does express RIPK1. Generally RIPK1 expresses in cytoplasm. cell membrane. Regarding which tissues have RIPK1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Uterus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 7538908, 15144186
Liver, Pubmed ID: 24275569
Umbilical vein endothelial cell, Pubmed ID: 8612133
Boster Scientific Support
Answered: 2019-09-12
Question
We are currently using anti-RIP/RIPK1 antibody PB9116 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
The anti-RIP/RIPK1 antibody (PB9116) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-03
Question
I see that the anti-RIP/RIPK1 antibody PB9116 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-01
Question
Do you have a BSA free version of anti-RIP/RIPK1 antibody PB9116 available?
A. Jha
Verified customer
Asked: 2019-05-17
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-RIP/RIPK1 antibody PB9116 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-17
Question
I was wanting to use your anti-RIP/RIPK1 antibody for WB for human umbilical vein endothelial cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human umbilical vein endothelial cell identification?
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
As indicated on the product datasheet, PB9116 anti-RIP/RIPK1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical vein endothelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-01
Question
I am looking for using your anti-RIP/RIPK1 antibody for regulation of necroptotic cell death studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
M. Jones
Verified customer
Asked: 2018-07-02
Answer
We appreciate your inquiry. This PB9116 anti-RIP/RIPK1 antibody is tested on jurkat whole cell lysate, 22rv1 whole cell lysate, hela whole cell lysate, a549 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-02
Question
Is a blocking peptide available for product anti-RIP/RIPK1 antibody (PB9116)?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
We do provide the blocking peptide for product anti-RIP/RIPK1 antibody (PB9116). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-31
Question
Does PB9116 anti-RIP/RIPK1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
It shows on the product datasheet, PB9116 anti-RIP/RIPK1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-12
Question
My question regarding product PB9116, anti-RIP/RIPK1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Banerjee
Verified customer
Asked: 2015-07-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9116 anti-RIP/RIPK1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-03
Question
you antibody to test anti-RIP/RIPK1 antibody PB9116 on human umbilical vein endothelial cell for research purposes, then I may be interested in using anti-RIP/RIPK1 antibody PB9116 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Wu
Verified customer
Asked: 2015-02-20
Answer
The products we sell, including anti-RIP/RIPK1 antibody PB9116, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-02-20
Question
We have observed staining in human brain. Do you have any suggestions? Is anti-RIP/RIPK1 antibody supposed to stain brain positively?
S. Walker
Verified customer
Asked: 2013-08-08
Answer
From literature brain does express RIPK1. From Uniprot.org, RIPK1 is expressed in right lobe of liver, umbilical vein endothelial cell, adrenal gland uterus, brain, leukemic t-cell, cervix carcinoma, erythroleukemia, liver, among other tissues. Regarding which tissues have RIPK1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Uterus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 7538908, 15144186
Liver, Pubmed ID: 24275569
Umbilical vein endothelial cell, Pubmed ID: 8612133
Boster Scientific Support
Answered: 2013-08-08
