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Key=meta_description, value=Boster Bio Anti-RIP3/RIPK3 Antibody catalog # PA2242. Tested in WB applications. This antibody reacts with Human. Supplied as 100μg/vial in Lyophilized form antibody.
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Lane 1: PANC Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: SKOV-3 Cell Lysate
Lane 4: M231 Cell Lysate
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Lane 1: PANC Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: SKOV-3 Cell Lysate
Lane 4: M231 Cell Lysate
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Lane 1: PANC Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: SKOV-3 Cell Lysate
Lane 4: M231 Cell Lysate
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Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Key=source_company, value=Boster PA2242
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=56887 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
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Key=research_category, value=Apoptosis, Associated Proteins, Cancer, Cell Biology, Cell Death, Immunology, Innate Immunity, Intracellular, Kinases, Protein Phosphorylation, Receptors, Ser/Thr Kinases, Signal Transduction, TLR Signaling
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Key=description, value=Boster Bio Anti-RIP3/RIPK3 Antibody catalog # PA2242. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-RIP3/RIPK3 Antibody catalog # PA2242. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-RIP3/RIPK3 Antibody
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Key=background, value=Receptor-interacting serine/threonine-protein kinase 3 (RIPK3), also known as RIP3 is an enzyme that in humans is encoded by the RIPK3 gene. This gene is mapped to 14q12. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
Key=gene_name, value=RIPK3
Key=synonyms, value=Receptor-interacting serine/threonine-protein kinase 3;2.7.11.1;RIP-like protein kinase 3;Receptor-interacting protein 3;RIP-3;RIPK3;RIP3;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Human

Key=subcellular_localization, value= Cytoplasm, cytosol . Cell membrane . Mitochondrion .
Key=sequence_similarities, value=Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Key=tissue_specificity, value= Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. .
Key=protein_function, value= Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. .
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 135170|sc 135171|sc 374639|sc 47368|sc 56228
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the N-terminus of human RIP3.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=56 kDa, 77 kDa
Key=custom_attribute_2, value=WB: PANC Cell, SW620 Cell, SKOV-3 Cell, M231 Cell
Key=custom_attribute_3, value=BA3486-2
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Anti-RIP3/RIPK3 Antibody Picoband®

RIPK3/RIP3 antibody

Boster Bio Anti-RIP3/RIPK3 Antibody catalog # PA2242. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: PA2242
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-RIP3/RIPK3 Antibody Picoband®

View all RIPK3/RIP3 Antibodies

SKU/Catalog Number

PA2242

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RIP3/RIPK3 Antibody catalog # PA2242. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RIP3/RIPK3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2242)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human RIP3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2242 is reactive to RIPK3 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

56 kDa, 77 kDa

Calculated molecular weight

56887 MW

Background of RIPK3/RIP3

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3), also known as RIP3 is an enzyme that in humans is encoded by the RIPK3 gene. This gene is mapped to 14q12. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA2242 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: PANC Cell, SW620 Cell, SKOV-3 Cell, M231 Cell

Validation Images & Assay Conditions

Gene/Protein Information For RIPK3 (Source: Uniprot.org, NCBI)

Gene Name

RIPK3

Full Name

Receptor-interacting serine/threonine-protein kinase 3

Weight

56887 MW

Superfamily

protein kinase superfamily

Alternative Names

Receptor-interacting serine/threonine-protein kinase 3;2.7.11.1;RIP-like protein kinase 3;Receptor-interacting protein 3;RIP-3;RIPK3;RIP3; RIPK3 RIP3 receptor interacting serine/threonine kinase 3 receptor-interacting serine/threonine-protein kinase 3|RIP-3|RIP-like protein kinase 3|receptor interacting protein 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RIPK3, check out the RIPK3 Infographic

RIPK3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RIPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2242 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Smad3-Targeted Therapy Protects against Cisplatin-Induced AKI by Attenuating Programmed Cell Death and Inflammation via a NOX4-Dependent Mechanism. Qin Yang,Li Gao,Xiao-wei Hu,Jia-nan Wang,Yao Zhang,Yu-hang Dong,Hui Yao Lan,Xiao-ming Meng
Species: Mouse
PA2242 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:NA

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4 Customer Q&As for Anti-RIP3/RIPK3 Antibody Picoband®

Question

We are currently using anti-RIP3/RIPK3 antibody PA2242 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

The anti-RIP3/RIPK3 antibody (PA2242) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-23

Question

Our team were happy with the WB result of your anti-RIP3/RIPK3 antibody. However we have observed positive staining in aortic endothelium fetal brain cytosol using this antibody. Is that expected? Could you tell me where is RIPK3 supposed to be expressed?

L. Miller

Verified customer

Asked: 2019-08-05

Answer

Based on literature, aortic endothelium fetal brain does express RIPK3. Generally RIPK3 expresses in cytoplasm, cytosol. Regarding which tissues have RIPK3 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, and Fetal brain, Pubmed ID: 10358032
Blood, Pubmed ID: 15489334
Cervix carcinoma, and Lymphocyte, Pubmed ID: 10339433
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-08-05

Question

We have been able to see staining in human blood. Any tips? Is anti-RIP3/RIPK3 antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

According to literature blood does express RIPK3. According to Uniprot.org, RIPK3 is expressed in blood, cervix carcinoma lymphocyte, aortic endothelium fetal brain, thalamus, among other tissues. Regarding which tissues have RIPK3 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, and Fetal brain, Pubmed ID: 10358032
Blood, Pubmed ID: 15489334
Cervix carcinoma, and Lymphocyte, Pubmed ID: 10339433
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-05-09

Question

We need using your anti-RIP3/RIPK3 antibody for thymus development studies. Has this antibody been tested with western blotting on sw620 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-08-25

Answer

Thanks for your inquiry. This PA2242 anti-RIP3/RIPK3 antibody is validated on panc cell lysate, sw620 cell lysate, m231 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-08-25

Order DetailsPrice
PA2242

100μg

$370
PA2242-10ug

10μg sample (liquid)

$99
PA2242-Biotin

100 μg Biotin conjugated

$570
PA2242-Cy3

100 μg Cy3 conjugated

$570
PA2242-Dylight488

100 μg Dylight488 conjugated

$570
PA2242-Dylight550

100 μg Dylight550 conjugated

$570
PA2242-Dylight594

100 μg Dylight594 conjugated

$570
PA2242-FITC

100 μg FITC conjugated

$570
PA2242-HRP

100 μg HRP conjugated

$570
PA2242-APC

100 μg APC conjugated

$670
PA2242-PE

100 μg PE conjugated

$670
PA2242-iFluor647

100 μg iFluor647 conjugated

$670
PA2242-carrier-free

Carrier Free

$370

More conjugates/formats

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PA2242
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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