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Product Info Summary
| SKU: | PA2051 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RIP/RIPK1 Antibody Picoband®
View all RIPK1/RIP1 Antibodies
SKU/Catalog Number
PA2051
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RIP/RIPK1 Antibody catalog # PA2051. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RIP/RIPK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2051)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human RIP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2051 is reactive to RIPK1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
76 kDa
Calculated molecular weight
75931 MW
Background of RIPK1/RIP1
RIPK1 (Regulator of G Protein Signaling 3), also called RIP, is an enzyme that in humans is encoded by the RIPK1 gene. Members of the TRAF protein family have been implicated in the activation of NF-kappa-B by the TNF superfamily. By yeast 2-hybrid and coimmunoprecipitation studies using mammalian cell extracts, Hsu et al. (1996) showed that RIP interacts with TRADD, TRAF1, TRAF2, and TRAF3. Hartz (2012) mapped the RIPK1 gene to chromosome 6p25.2 based on an alignment of the RIPK1 sequence with the genomic sequence. Stanger et al. (1995) found that overexpression of Rip in mammalian cells induced morphologic changes characteristic of apoptosis. They suggested that RIP may be an important element in the signal transduction machinery that mediates programmed cell death.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2051 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human K562 whole cell, human Hela whole cell, human Raji whole cell, human Jurkat whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RIPK1 using anti-RIPK1 antibody (PA2051).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIPK1 antigen affinity purified polyclonal antibody (Catalog # PA2051) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIPK1 at approximately 76 kDa. The expected band size for RIPK1 is at 76 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RIPK1 (Source: Uniprot.org, NCBI)
Gene Name
RIPK1
Full Name
Receptor-interacting serine/threonine-protein kinase 1
Weight
75931 MW
Superfamily
protein kinase superfamily
Alternative Names
Receptor-interacting serine/threonine-protein kinase 1;2.7.11.1;Cell death protein RIP;Receptor-interacting protein 1;RIP-1;Serine/threonine-protein kinase RIP;RIPK1;RIP, RIP1; RIPK1 AIEFL, IMD57, RIP, RIP-1, RIP1 receptor interacting serine/threonine kinase 1 receptor-interacting serine/threonine-protein kinase 1|cell death protein RIP|receptor (TNFRSF)-interacting serine-threonine kinase 1|receptor-interacting protein 1|receptor-interacting protein kinase 1|serine/threonine-protein kinase RIP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIPK1, check out the RIPK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RIP/RIPK1 Antibody Picoband® (PA2051)
Hello CJ!
PA2051 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors
Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-RIP/RIPK1 Antibody Picoband®
Question
Can you help my question with product PA2051, anti-RIP/RIPK1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2051 anti-RIP/RIPK1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-16
Question
Would anti-RIP/RIPK1 antibody PA2051 work on dog WB with adrenal gland uterus?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
Our lab technicians have not tested anti-RIP/RIPK1 antibody PA2051 on dog. You can run a BLAST between dog and the immunogen sequence of anti-RIP/RIPK1 antibody PA2051 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog adrenal gland uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-26
Question
Please see the WB image, lot number and protocol we used for right lobe of liver using anti-RIP/RIPK1 antibody PA2051. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-06
Question
Do you have a BSA free version of anti-RIP/RIPK1 antibody PA2051 available?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-RIP/RIPK1 antibody PA2051 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-27
Question
We have been able to see staining in human right lobe of liver. What should we do? Is anti-RIP/RIPK1 antibody supposed to stain right lobe of liver positively?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
From what I have seen in literature right lobe of liver does express RIPK1. From what I have seen in Uniprot.org, RIPK1 is expressed in right lobe of liver, umbilical vein endothelial cell, adrenal gland uterus, brain, leukemic t-cell, cervix carcinoma, erythroleukemia, liver, among other tissues. Regarding which tissues have RIPK1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Uterus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 7538908, 15144186
Liver, Pubmed ID: 24275569
Umbilical vein endothelial cell, Pubmed ID: 8612133
Boster Scientific Support
Answered: 2020-01-03
Question
My team were happy with the WB result of your anti-RIP/RIPK1 antibody. However we have observed positive staining in right lobe of liver cytoplasm. cell membrane. using this antibody. Is that expected? Could you tell me where is RIPK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
Based on literature, right lobe of liver does express RIPK1. Generally RIPK1 expresses in cytoplasm. cell membrane. Regarding which tissues have RIPK1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Uterus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 7538908, 15144186
Liver, Pubmed ID: 24275569
Umbilical vein endothelial cell, Pubmed ID: 8612133
Boster Scientific Support
Answered: 2019-12-18
Question
you antibody using your anti-RIP/RIPK1 antibody for immunodeficiency 57 (imd57) studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We appreciate your inquiry. This PA2051 anti-RIP/RIPK1 antibody is validated on hela whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-23
Question
Will PA2051 anti-RIP/RIPK1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
As indicated on the product datasheet, PA2051 anti-RIP/RIPK1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-12
Question
I see that the anti-RIP/RIPK1 antibody PA2051 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-29
Question
I was wanting to use your anti-RIP/RIPK1 antibody for WB for human right lobe of liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right lobe of liver identification?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
It shows on the product datasheet, PA2051 anti-RIP/RIPK1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-26
Question
We are currently using anti-RIP/RIPK1 antibody PA2051 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The anti-RIP/RIPK1 antibody (PA2051) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-20
Question
Would anti-RIP/RIPK1 antibody PA2051 work for WB with right lobe of liver?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
According to the expression profile of right lobe of liver, RIPK1 is highly expressed in right lobe of liver. So, it is likely that anti-RIP/RIPK1 antibody PA2051 will work for WB with right lobe of liver.
Boster Scientific Support
Answered: 2019-06-12
Question
Is this PA2051 anti-RIP/RIPK1 antibody reactive to the isotypes of RIPK1?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
The immunogen of PA2051 anti-RIP/RIPK1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human RIP(411-425aa RRRRVSHDPFAQQRP). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-RIP/RIPK1 antibody PA2051. Let me know if you need anything else.
R. Banerjee
Verified customer
Asked: 2018-08-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-09
Question
I would like to test anti-RIP/RIPK1 antibody PA2051 on human right lobe of liver for research purposes, then I may be interested in using anti-RIP/RIPK1 antibody PA2051 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Anderson
Verified customer
Asked: 2018-06-28
Answer
The products we sell, including anti-RIP/RIPK1 antibody PA2051, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-28
Question
Is a blocking peptide available for product anti-RIP/RIPK1 antibody (PA2051)?
J. Johnson
Verified customer
Asked: 2016-07-05
Answer
We do provide the blocking peptide for product anti-RIP/RIPK1 antibody (PA2051). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-07-05
