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Product Info Summary
| SKU: | M00767 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-RBPJK Monoclonal Antibody
SKU/Catalog Number
M00767
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RBPJK Monoclonal Antibody catalog # M00767. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RBPJK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00767)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEEF-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RBPJK Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00767 is reactive to RBPJ in Human, Mouse, Rat
Applications
M00767 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
15 kDa
Calculated molecular weight
67820 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of RBPJK expression in MCF7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RBPJ (Source: Uniprot.org, NCBI)
Gene Name
RBPJ
Full Name
Recombining binding protein suppressor of hairless
Weight
67820 MW
Superfamily
Su(H) family
Alternative Names
CBF1; CBF-1; CSL; H-2K binding factor-2; IGKJRB1; IGKJRB1RBPSUHCBF1; IGKJRBRBP-J kappa; immunoglobulin kappa J region recombination signal binding protein 1; J kappa-recombination signal-binding protein; KBF2; RBPJ; RBP-JK; RBPJKMGC61669; RBP-Jrecombining binding protein suppressor of hairless (Drosophila); RBPSUH; recombination signal binding protein for immunoglobulin kappa J region; recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; SUH; suppressor of hairless homolog RBPJ AOS3, CBF-1, CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBP-J kappa, RBP-JKK, RBPSUH, SUH, csl, RBPJ recombination signal binding protein for immunoglobulin kappa J region recombining binding protein suppressor of hairless|H-2K binding factor-2|immunoglobulin kappa J region recombination signal binding protein 1|renal carcinoma antigen NY-REN-30|suppressor of hairless homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBPJ, check out the RBPJ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBPJ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RBPJK Monoclonal Antibody (M00767)
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No publications found for M00767
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Customer Q&As
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4 Customer Q&As for Anti-RBPJK Monoclonal Antibody
Question
I was wanting to use your anti-RBPJK Monoclonal antibody for WB for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
As indicated on the product datasheet, M00767 anti-RBPJK Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-07
Question
Will anti-RBPJK Monoclonal antibody M00767 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
According to the expression profile of placenta, RBPJ is highly expressed in placenta. So, it is likely that anti-RBPJK Monoclonal antibody M00767 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-05-31
Question
We are currently using anti-RBPJK Monoclonal antibody M00767 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
G. Jha
Verified customer
Asked: 2017-03-09
Answer
The anti-RBPJK Monoclonal antibody (M00767) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-09
Question
I see that the anti-RBPJK Monoclonal antibody M00767 works with WB, what is the protocol used to produce the result images on the product page?
A. Gonzalez
Verified customer
Asked: 2015-03-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-03-20
