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Product Info Summary
| SKU: | M00767-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-RBPJK/RBPJ Picoband® Antibody (monoclonal, 6G4)
SKU/Catalog Number
M00767-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RBPJK/RBPJ Picoband® Antibody (monoclonal, 6G4) catalog # M00767-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RBPJK/RBPJ Picoband® Antibody (monoclonal, 6G4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00767-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6G4
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human RBPJK/RBPJ recombinant protein (Position: K41-Q467).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00767-1 is reactive to RBPJ in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
55.637kDa
Background of csl/RBPJK
Recombination signal binding protein for immunoglobulin kappa J region is a protein that in humans is encoded by the RBPJ gene. It is mapped to 4p15.2. The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00767-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse
Positive Control
WB: human HEK293 whole cell, human HEPG2 whole cell, human HELA whole cell, human MCF-7 whole cell, human U87 whole cell, human HL-60 whole cell, rat NRK whole cell, mouse smooth muscle tissue
FCM: HEPA1-6 cell, U937 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RBPJK/RBPJ using anti-RBPJK/RBPJ antibody (M00767-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human HEPG2 whole cell lysates,
Lane 3: human HELA whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human U87 whole cell lysates,
Lane 6: human HL-60 whole cell lysates,
Lane 7: rat NRK whole cell lysates,
Lane 8: mouse smooth muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RBPJK/RBPJ antigen affinity purified monoclonal antibody (Catalog # M00767-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RBPJK/RBPJ at approximately 56KD. The expected band size for RBPJK/RBPJ is at 56KD.
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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-RBPJK/RBPJ antibody (M00767-1).
Overlay histogram showing HEPA1-6 cells stained with M00767-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- RBPJK/RBPJ Antibody (M00767-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of U937 cells using anti-RBPJK/RBPJ antibody (M00767-1).
Overlay histogram showing U937 cells stained with M00767-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- RBPJK/RBPJ Antibody (M00767-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RBPJ (Source: Uniprot.org, NCBI)
Gene Name
RBPJ
Full Name
Recombining binding protein suppressor of hairless
Weight
55.637kDa
Superfamily
Su(H) family
Alternative Names
SRK; STD; TZK; ZAP70; ZAP-70; ZAP 70; P43403 RBPJ AOS3, CBF-1, CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBP-J kappa, RBP-JKK, RBPSUH, SUH, csl, RBPJ recombination signal binding protein for immunoglobulin kappa J region recombining binding protein suppressor of hairless|H-2K binding factor-2|immunoglobulin kappa J region recombination signal binding protein 1|renal carcinoma NY-REN-30|suppressor of hairless homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBPJ, check out the RBPJ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBPJ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RBPJK/RBPJ Picoband® Antibody (monoclonal, 6G4) (M00767-1)
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