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Product Info Summary
| SKU: | M02846-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | IHC-P, ICC, WB |
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Product info
Product Name
Anti-PSMA Purified FOLH1 Monoclonal Antibody
View all PSMA/FOLH1/NAALADase I Antibodies
SKU/Catalog Number
M02846-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PSMA Purified FOLH1 Monoclonal Antibody (Catalog# M02846-2). Tested in WB, IHC-P, ICC application(s). This antibody reacts with Pig, Mouse, Rat, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-PSMA Purified FOLH1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02846-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
GCP-04
Isotype
Mouse IgG1
Immunogen
Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells. The mouse monoclonal antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02846-2 is reactive to FOLH1 in Human, Mouse, Pig, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
84331 MW
Background of PSMA/FOLH1/NAALADase I
Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02846-2 is guaranteed for IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1 μg/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of FOLH1 using Anti-PSMA Purified FOLH1 Monoclonal Antibody (M02846-2).
Immunohistochemistry staining of GCPII in human medulla oblongata by GCP-04 monoclonal antibody. Mag. 40x; positive astrocytes in white matter.For more protocol information of IHC
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Figure 3. Immunohistochemistry validation of FOLH1 using Anti-PSMA Purified FOLH1 Monoclonal Antibody (M02846-2).
Immunohistochemistry staining of GCPII in human prostate by GCP-04 monoclonal antibody. Mag. 400x; positive epithelium of the prostate glands.For more protocol information of IHC
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Figure 4. Immunohistochemistry validation of FOLH1 using Anti-PSMA Purified FOLH1 Monoclonal Antibody (M02846-2).
Immunohistochemistry staining of GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-PSMA Purified FOLH1 Monoclonal Antibody M02846-2
Western blotting analysis of a fragment of human GCPII (aminoacids 44-750) produced in S2 cells by GCP-04 monoclonal antibody. Lanes 1Protein Target Info & Infographic
Gene/Protein Information For FOLH1 (Source: Uniprot.org, NCBI)
Gene Name
FOLH1
Full Name
Glutamate carboxypeptidase 2
Weight
84331 MW
Superfamily
peptidase M28 family
Alternative Names
GCP2, FOLH1, NAALADase I, PGGCP, FGGCP, FGCP, FOLH1 FGCP, FOLH, GCP2, GCPII, NAALAD1, NAALAdase, PSM, PSMA, mGCP folate hydrolase 1 glutamate carboxypeptidase 2|N-acetylated alpha-linked acidic dipeptidase 1|N-acetylated-alpha-linked acidic dipeptidase I|NAALADase I|cell growth-inhibiting gene 27 protein|folylpoly-gamma-glutamate carboxypeptidase|glutamate carboxylase II|glutamate carboxypeptidase II|membrane glutamate carboxypeptidase|prostate specific membrane variant F|pteroylpoly-gamma-glutamate carboxypeptidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOLH1, check out the FOLH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOLH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSMA Purified FOLH1 Monoclonal Antibody (M02846-2)
Hello CJ!
M02846-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ability to Suppress TGF-β-Activated Myofibroblast Differentiation Distinguishes the Anti-pulmonary Fibrosis Efficacy of Two Danshen-Containing Chinese Herbal Medicine Prescriptions Rui Shao,1,2,† Fu-jiang Wang,1,2,† Ming Lyu,1,2,‡ Jian Yang,1,2 Peng Zha
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