Anti-CDC2 (Ab-15) CDK1 Antibody

CDC2/CDK1 antibody

Boster Bio Anti-CDC2 (Ab-15) CDK1 Antibody (Catalog # A00209). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: A00209
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-CDC2 (Ab-15) CDK1 Antibody

View all CDC2/CDK1 Antibodies

SKU/Catalog Number

A00209

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-CDC2 (Ab-15) CDK1 Antibody (Catalog # A00209). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDC2 (Ab-15) CDK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00209)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa. 13~17 (G-T-Y-G-V) derived from Human CDC2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00209 is reactive to CDK1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

265351 MW

Background of CDC2/CDK1

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Y Gu, et al. (1992) EMBO J. 11(11): 3995

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00209 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 34kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

Gene/Protein Information For CDK1 (Source: Uniprot.org, NCBI)

Gene Name

CDK1

Full Name

Cyclin-dependent kinase 1

Weight

265351 MW

Superfamily

protein kinase superfamily

Alternative Names

CREB-binding protein;2.3.1.48 ;CREBBP;CBP; CDK1 CDC2, CDC28A, P34CDC2 cyclin dependent kinase 1 cyclin-dependent kinase 1|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDK1, check out the CDK1 Infographic

CDK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00209 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells

Human enterovirus 68 interferes with the host cell cycle to facilitate viral production

Coxsackievirus A6 induces cell cycle arrest in G0/G1 phase for viral production

Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats

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5 Customer Q&As for Anti-CDC2 (Ab-15) CDK1 Antibody

Question

We are currently using anti-CDC2 (Ab-15) antibody A00209 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

The anti-CDC2 (Ab-15) antibody (A00209) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-20

Question

Our lab used your anti-CDC2 (Ab-15) antibody for IF on cervix carcinoma a few months ago. I am using human, and We intend to use the antibody for WB next. My lab would like examining cervix carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-10-10

Answer

I viewed the website and datasheets of our anti-CDC2 (Ab-15) antibody and it seems that A00209 has been validated on human in both IF and WB. Thus A00209 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-10

Question

We were happy with the WB result of your anti-CDC2 (Ab-15) antibody. However we have been able to see positive staining in skin nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-10-26

Answer

Based on literature, skin does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-10-26

Question

I was wanting to use using your anti-CDC2 (Ab-15) antibody for protein deubiquitination studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

S. Moore

Verified customer

Asked: 2016-11-28

Answer

We appreciate your inquiry. This A00209 anti-CDC2 (Ab-15) antibody is validated on hela cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-11-28

Question

We have seen staining in human leukemic t-cell. Do you have any suggestions? Is anti-CDC2 (Ab-15) antibody supposed to stain leukemic t-cell positively?

J. Jackson

Verified customer

Asked: 2013-10-11

Answer

Based on literature leukemic t-cell does express CDK1. Based on Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-10-11

Order DetailsPrice
A00209

100ul

$360

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