Product Info Summary
SKU: | A00209 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-CDC2 (Ab-15) CDK1 Antibody
SKU/Catalog Number
A00209
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CDC2 (Ab-15) CDK1 Antibody (Catalog # A00209). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC2 (Ab-15) CDK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00209)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 13~17 (G-T-Y-G-V) derived from Human CDC2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00209 is reactive to CDK1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
265351 MW
Background of CDC2/CDK1
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Y Gu, et al. (1992) EMBO J. 11(11): 3995
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00209 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 34kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CDK1 using anti-CDK1 antibody (A00209).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 antigen affinity purified polyclonal antibody (Catalog # A00209) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CDK1.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using CDC2(Ab-15) Antibody #A00209.
Click image to see more details
Figure 2. IHC analysis of CDK1 using anti-CDK1 antibody (A00209).
CDK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (A00209) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CDK1 (Source: Uniprot.org, NCBI)
Gene Name
CDK1
Full Name
Cyclin-dependent kinase 1
Weight
265351 MW
Superfamily
protein kinase superfamily
Alternative Names
CREB-binding protein;2.3.1.48 ;CREBBP;CBP; CDK1 CDC2, CDC28A, P34CDC2 cyclin dependent kinase 1 cyclin-dependent kinase 1|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK1, check out the CDK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDC2 (Ab-15) CDK1 Antibody (A00209)
Hello CJ!
A00209 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells
Human enterovirus 68 interferes with the host cell cycle to facilitate viral production
Coxsackievirus A6 induces cell cycle arrest in G0/G1 phase for viral production
Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats
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Customer Q&As
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5 Customer Q&As for Anti-CDC2 (Ab-15) CDK1 Antibody
Question
We are currently using anti-CDC2 (Ab-15) antibody A00209 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
The anti-CDC2 (Ab-15) antibody (A00209) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-20
Question
Our lab used your anti-CDC2 (Ab-15) antibody for IF on cervix carcinoma a few months ago. I am using human, and We intend to use the antibody for WB next. My lab would like examining cervix carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
I viewed the website and datasheets of our anti-CDC2 (Ab-15) antibody and it seems that A00209 has been validated on human in both IF and WB. Thus A00209 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-10-10
Question
We were happy with the WB result of your anti-CDC2 (Ab-15) antibody. However we have been able to see positive staining in skin nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
Based on literature, skin does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-10-26
Question
I was wanting to use using your anti-CDC2 (Ab-15) antibody for protein deubiquitination studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
S. Moore
Verified customer
Asked: 2016-11-28
Answer
We appreciate your inquiry. This A00209 anti-CDC2 (Ab-15) antibody is validated on hela cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-11-28
Question
We have seen staining in human leukemic t-cell. Do you have any suggestions? Is anti-CDC2 (Ab-15) antibody supposed to stain leukemic t-cell positively?
J. Jackson
Verified customer
Asked: 2013-10-11
Answer
Based on literature leukemic t-cell does express CDK1. Based on Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-10-11