Anti-Phospho-Tyrosine Hydroxylase (Ser31) Antibody

Product Name

Anti-Phospho-Tyrosine Hydroxylase (Ser31) Antibody

View all Tyrosine Hydroxylase Antibodies

SKU/Catalog Number

P01917

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Tyrosine Hydroxylase (Ser31) Antibody (Catalog # P01917). Tested in WB, IHC, ICC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Tyrosine Hydroxylase (Ser31) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01917)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01917 is reactive to TH in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

52 kDa

Calculated molecular weight

55966 MW

Background of Tyrosine Hydroxylase

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

P01917 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:300-1:1000
ICC: 1:50-1:300

Validation Images & Assay Conditions

Gene/Protein Information For TH (Source: Uniprot.org, NCBI)

Alternative Names

Dystonia 14 antibody, DYT14 antibody, DYT5b antibody, EC 1.14.16.2 antibody, OTTHUMP00000011225 antibody, OTTHUMP00000011226 antibody, ple antibody, Protein Pale antibody, TH antibody, The antibody, TY3H_HUMAN antibody, TYH antibody, Tyrosine 3 hydroxylase antibody, Tyrosine 3 monooxygenase antibody, Tyrosine 3-hydroxylase antibody, Tyrosine 3-monooxygenase antibody, Tyrosine hydroxylase antibody TH DYT14, DYT5b, TYH tyrosine hydroxylase tyrosine 3-monooxygenase|dystonia 14|tyrosine 3-hydroxylase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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