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Product Info Summary
| SKU: | A03459-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Dab1 (Ab-232) Antibody
SKU/Catalog Number
A03459-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Dab1 (Ab-232) Antibody (Catalog # A03459-1). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dab1 (Ab-232) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03459-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.230~234 (G-V-Y-D-V) derived from Human Dab1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03459-1 is reactive to DAB1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
16962 MW
Background of DAB1
The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.
Kelian Chen, et,al. (2003) J. Cell Sci ; 117: 4527 - 4536.
Vera Strasser, et,al. (2004) Mol. Cell. Biol ; 24: 1378 - 1386.
Izhar Ben-Shlomo, et,al. (2003) Sci. STKE ; 2003: 9.
H. M. Kim, et,al. (2002) PNAS ; 99: 4020.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03459-1 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 80kd
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Figure 1. IHC analysis of DAB1 using anti-DAB1 antibody (A03459-1).
DAB1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DAB1 Antibody (A03459-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For DAB1 (Source: Uniprot.org, NCBI)
Gene Name
DAB1
Full Name
Disabled homolog 1
Weight
16962 MW
Alternative Names
Interleukin-36 receptor antagonist protein;IL-36Ra ;FIL1 delta;IL-1-related protein 3;IL-1RP3;Interleukin-1 HY1;IL-1HY1;Interleukin-1 delta;IL-1 delta;Interleukin-1 family member 5;IL-1F5;Interleukin-1 receptor antagonist homolog 1;IL-1ra homolog 1;Interleukin-1-like protein 1;IL-1L1;IL36RN;FIL1D, IL1F5, IL1HY1, IL1L1, IL1RP3;UNQ1896/PRO4342; DAB1 SCA37 DAB adaptor protein 1 disabled homolog 1|DAB1, reelin adaptor protein|Dab reelin signal transducer 1|Dab, reelin signal transducer, homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAB1, check out the DAB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dab1 (Ab-232) Antibody (A03459-1)
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No publications found for A03459-1
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Customer Q&As
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1 Customer Q&As for Anti-Dab1 (Ab-232) Antibody
Question
We are currently using anti-Dab1 (Ab-232) antibody A03459-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-Dab1 (Ab-232) antibody (A03459-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
