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Product Info Summary
| SKU: | PA2087 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Prolactin Receptor/PRLR Antibody Picoband®
View all Prolactin R Antibodies
SKU/Catalog Number
PA2087
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prolactin Receptor/PRLR Antibody catalog # PA2087. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prolactin Receptor/PRLR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2087)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse PRLR, identical to the related rat sequence, and different from the related human sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2087 is reactive to Prlr in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated molecular weight
68241 MW
Background of Prolactin R
PRLR (Prolactin Receptor), is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al. (1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al. (1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues (histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR (conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2087 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat PC-12 whole cell, human Hela whole cell, human MCF-7 whole cell
IHC: Human Mammary Cancer Tissue, Rat Testis Tissue
Validation Images & Assay Conditions
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Anti-PRLR antibody, PA2087, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-PRLR antibody, PA2087, IHC(P)
IHC(P): Rat Testis Tissue
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Figure 1. Western blot analysis of PRLR using anti-PRLR antibody (PA2087).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody (Catalog # PA2087) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRLR at approximately 90KD. The expected band size for PRLR is at 70KD.
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Figure 4. Western blot analysis of PRLR using anti-PRLR antibody (PA2087).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody (Catalog # PA2087) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRLR at approximately 95KD. The expected band size for PRLR is at 70KD.
Protein Target Info & Infographic
Gene/Protein Information For Prlr (Source: Uniprot.org, NCBI)
Gene Name
Prlr
Full Name
Prolactin receptor
Weight
68241 MW
Superfamily
type I cytokine receptor family
Alternative Names
Prolactin receptor;PRL-R;Prlr; PRLR HPRL, MFAB, RI-PRLR, hPRLrI prolactin receptor prolactin receptor|hPRL receptor|secreted prolactin binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Prlr, check out the Prlr Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Prlr: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prolactin Receptor/PRLR Antibody Picoband® (PA2087)
Hello CJ!
PA2087 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
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Customer Q&As
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4 Customer Q&As for Anti-Prolactin Receptor/PRLR Antibody Picoband®
Question
I was wanting to use your anti-Prolactin Receptor/PRLR antibody for IHC-P for human mammary carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mammary carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
You can see on the product datasheet, PA2087 anti-Prolactin Receptor/PRLR antibody has been tested for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human mammary carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-19
Question
Is a blocking peptide available for product anti-Prolactin Receptor/PRLR antibody (PA2087)?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
We do provide the blocking peptide for product anti-Prolactin Receptor/PRLR antibody (PA2087). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-31
Question
Please see the WB image, lot number and protocol we used for mammary carcinoma using anti-Prolactin Receptor/PRLR antibody PA2087. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-18
Question
Is there a BSA free version of anti-Prolactin Receptor/PRLR antibody PA2087 available?
J. Roberts
Verified customer
Asked: 2018-05-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Prolactin Receptor/PRLR antibody PA2087 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-01
