Anti-ErbB 4/ERBB4 Antibody Picoband®

Product Name

Anti-ErbB 4/ERBB4 Antibody Picoband®

View all ErbB4/Her4 Antibodies

SKU/Catalog Number

PA1881

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ErbB 4/ERBB4 Antibody catalog # PA1881. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ErbB 4/ERBB4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1881)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 4, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1881 is reactive to ERBB4 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

180 kDa

Calculated molecular weight

146808 MW

Background of ErbB4/Her4

ERBB4 (V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4) also known as ONCOGENE ERBB4 or HER4, is an enzyme that in humans is encoded by the ERBB4 gene. The HER4/ERBB4 gene is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2 and ERBB3. The ERBB4 gene is mapped on 2q34. ERBB4 is a single-pass type I transmembrane protein with multiple furin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domainbinding motif. Huang et al. (2000) reported that ERBB4 is enriched in the postsynaptic density and associates with PSD95. ERBB4 is a transmembrane receptor tyrosine kinase that regulates cell proliferation and differentiation. After binding its ligand, heregulin, or activation of protein kinase C by TPA, the ERBB4 ectodomain is cleaved by a metalloprotease. Ni et al. (2001)concluded that gamma-secretase cleavage of ERBB4 may represent another mechanism for receptor tyrosine kinase-mediated signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1881 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: A549 Cell, HELA Cell, HELA Cell, U87 Cell, NEURO Cell

Validation Images & Assay Conditions

Gene/Protein Information For ERBB4 (Source: Uniprot.org, NCBI)

Alternative Names

Receptor tyrosine-protein kinase erbB-4;2.7.10.1;Proto-oncogene-like protein c-ErbB-4;Tyrosine kinase-type cell surface receptor HER4;p180erbB4;ERBB4 intracellular domain;4ICD;E4ICD;s80HER4;ERBB4;HER4; ERBB4 ALS19, HER4, p180erbB4 erb-b2 receptor tyrosine kinase 4 receptor tyrosine-protein kinase erbB-4|avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4|human epidermal growth factor receptor 4|proto-oncogene-like protein c-ErbB-4|tyrosine kinase-type cell surface receptor HER4|v-erb-a erythroblastic leukemia viral oncogene homolog 4|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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