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Product Info Summary
| SKU: | P00007-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00007-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody catalog # P00007-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00007-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IFA-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human STAT3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00007-2 is reactive to STAT3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
88 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00007-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:100
ICC/IF 1:50-1:000
IP 1:30
Positive Control
IHC: human pancreas tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STAT3 using anti-STAT3 antibody (P00007-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse NIH/3T3 whole cell lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified monoclonal antibody (Catalog # P00007-2) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
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Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-STAT3 (Y705) Antibody.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of HeLa cells treated with IFN-alpha, using Phospho-STAT3 (Y705) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For STAT3 (Source: Uniprot.org, NCBI)
Gene Name
STAT3
Full Name
Signal transducer and activator of transcription 3
Weight
88068 MW
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; STAT3 ADMIO, ADMIO1, APRF, HIES signal transducer and activator of transcription 3 signal transducer and activator of transcription 3|DNA-binding protein APRF|acute-phase response factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT3, check out the STAT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody (P00007-2)
Hello CJ!
P00007-2 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
He J,Zhang W,Di T,Meng J,Qi Y,Li G,Zhang Y,Su H,Yan W.Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody.Biomed Pharmacother.2020
Species: Human,Mouse
P00007-2 usage in article: APP:WB, SAMPLE:HOS CELL AND K7M2 CELL, DILUTION:1:500
Protective effects of Tongxinluo on cerebral ischemia/reperfusion injury related to Connexin 43/Calpain II/Bax/Caspase-3 pathway in rat
IL-6 Inhibits Starvation-induced Autophagy via the STAT3/Bcl-2 Signaling Pathway
Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuatedSalmonellaserovartyphimuriumfor prostate cancer treatment
Prognostic role of STAT3 in solid tumors: a systematic review and meta-analysis
Hu T, Zhang C, Tang Q, Su Y, Li B, Chen L, Zhang Z, Cai T, Zhu Y. Bmc Cancer. 2013 May 22;13:251. Doi: 10.1186/1471-2407-13-251. Variant G6Pd Levels Promote Tumor Cell Proliferation Or Apoptosis Via The Stat3/5 Pathway In The Human Melanoma Xenogr...
Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Question
you antibody using your anti-Phospho-STAT3 (Y705) Rabbit Monoclonal antibody for signaling by leptin studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
K. Carter
Verified customer
Asked: 2019-12-05
Answer
I appreciate your inquiry. This P00007-2 anti-Phospho-STAT3 (Y705) Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-05
Question
Our lab were content with the WB result of your anti-Phospho-STAT3 (Y705) Rabbit Monoclonal antibody. However we have seen positive staining in placenta cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is STAT3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
Based on literature, placenta does express STAT3. Generally STAT3 expresses in cytoplasm. nucleus. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451
Boster Scientific Support
Answered: 2019-08-05
Question
We have tried in the past anti-Phospho-STAT3 (Y705) Rabbit Monoclonal antibody for IHC on liver a few years ago. I am using mouse, and We intend to use the antibody for WB next. We are interested in examining liver as well as kidney pancreas in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
I have checked the website and datasheets of our anti-Phospho-STAT3 (Y705) Rabbit Monoclonal antibody and it appears that P00007-2 has been validated on mouse in both IHC and WB. Thus P00007-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-02
Question
We have observed staining in mouse liver. What should we do? Is anti-Phospho-STAT3 (Y705) Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
From literature liver does express STAT3. From Uniprot.org, STAT3 is expressed in upper lobe of lung, placenta, kidney pancreas, liver, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451
Boster Scientific Support
Answered: 2018-02-21
Question
We are currently using anti-Phospho-STAT3 (Y705) Rabbit Monoclonal antibody P00007-2 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
A. Bhatt
Verified customer
Asked: 2015-04-07
Answer
The anti-Phospho-STAT3 (Y705) Rabbit Monoclonal antibody (P00007-2) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-07
