Product Info Summary
SKU: | A02822-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PPP2R1A Antibody Picoband®
SKU/Catalog Number
A02822-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PPP2R1A Antibody Picoband® catalog # A02822-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PPP2R1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02822-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
A synthetic peptide corresponding to a sequence in the middle region of human PPP2R1A, which shares 95.2% amino acid (aa) sequence identity with mouse PPP2R1A, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02822-2 is reactive to PPP2R1A in Human, Monkey, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
96950 MW
Background of PPP2R1A
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform is an enzyme that in humans is encoded by the PPP2R1A gene. This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02822-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human A431 whole cell, monkey COS-7 whole cell, rat kidney tissue, rat C6 whole cell, mouse NIH/3T3 whole cell
IHC: human liver cancer tissue, human lung cancer tissue
ICC/IF: A549 cell
FCM: 293T cell, C6 cell, RAW2647 cell
Validation Images & Assay Conditions
![a02822 2 ppp2r1a primary antibodies fcm testing 5_1 a02822 2 ppp2r1a primary antibodies fcm testing 5_1](https://www.bosterbio.com/media/catalog/product/a/0/a02822-2-ppp2r1a-primary-antibodies-fcm-testing-5_1.png)
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Figure 5. Flow Cytometry analysis of 293T cells using anti-PPP2R1A antibody (A02822-2).
Overlay histogram showing 293T cells stained with A02822-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R1A Antibody (A02822-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a02822 2 ppp2r1a primary antibodies wb testing 1_1 a02822 2 ppp2r1a primary antibodies wb testing 1_1](https://www.bosterbio.com/media/catalog/product/a/0/a02822-2-ppp2r1a-primary-antibodies-wb-testing-1_1.jpg)
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Figure 1. Western blot analysis of PPP2R1A using anti-PPP2R1A antibody (A02822-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R1A antigen affinity purified polyclonal antibody (Catalog # A02822-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R1A at approximately 65 kDa. The expected band size for PPP2R1A is at 65 kDa.
![a02822 2 ppp2r1a primary antibodies fcm testing 6 a02822 2 ppp2r1a primary antibodies fcm testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a02822-2-ppp2r1a-primary-antibodies-fcm-testing-6.png)
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Figure 6. Flow Cytometry analysis of C6 cells using anti-PPP2R1A antibody (A02822-2).
Overlay histogram showing C6 cells stained with A02822-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R1A Antibody (A02822-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a02822 2 ppp2r1a primary antibodies ihc testing 2 a02822 2 ppp2r1a primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a02822-2-ppp2r1a-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of PPP2R1A using anti-PPP2R1A antibody (A02822-2).
PPP2R1A was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2R1A Antibody (A02822-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a02822 2 ppp2r1a primary antibodies fcm testing 7 a02822 2 ppp2r1a primary antibodies fcm testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a02822-2-ppp2r1a-primary-antibodies-fcm-testing-7.png)
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Figure 7. Flow Cytometry analysis of RAW264.7 cells using anti-PPP2R1A antibody (A02822-2).
Overlay histogram showing RAW264.7 cells stained with A02822-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R1A Antibody (A02822-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a02822 2 ppp2r1a primary antibodies ihc testing 3 a02822 2 ppp2r1a primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a02822-2-ppp2r1a-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of PPP2R1A using anti-PPP2R1A antibody (A02822-2).
PPP2R1A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2R1A Antibody (A02822-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a02822 2 ppp2r1a primary antibodies if testing 4 a02822 2 ppp2r1a primary antibodies if testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a02822-2-ppp2r1a-primary-antibodies-if-testing-4.jpg)
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Figure 4. IF analysis of PPP2R1A using anti-PPP2R1A antibody (A02822-2).
PPP2R1A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP2R1A Antibody (A02822-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PPP2R1A (Source: Uniprot.org, NCBI)
Gene Name
PPP2R1A
Full Name
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Weight
96950 MW
Superfamily
phosphatase 2A regulatory subunit A family
Alternative Names
Medium tumor antigen-associated 61 kDa protein; MGC786; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PP2AAALPHA; PP2A-Aalpha; PR65A; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alphaisoform; protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform; protein phosphatase 2, regulatory subunit A, alpha; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alphaisoform; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alphaisoform PPP2R1A MRD36, PP2A-Aalpha, PP2AA, PP2AAALPHA, PR65A protein phosphatase 2 scaffold subunit Aalpha serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform|medium tumor antigen-associated 61 KDA protein|protein phosphatase 2, regulatory subunit A, alpha|testicular secretory protein Li 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP2R1A, check out the PPP2R1A Infographic
![PPP2R1A infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP2R1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP2R1A Antibody Picoband® (A02822-2)
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