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Product Info Summary
| SKU: | A00109-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PD-L1/Cd274 Antibody Picoband®
SKU/Catalog Number
A00109-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PD-L1/Cd274 Antibody Picoband® catalog # A00109-3. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PD-L1/Cd274 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00109-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse PD-L1/Cd274 recombinant protein (Position: E45-H239).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00109-3 is reactive to Cd274 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
33275 MW
Background of PD-L1
Programmed death-ligand 1 (PD-L1), also known as CD274 or B7-H1, is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. Additionally, PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What’s more, it has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00109-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: mouse thymus tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PD-L1/Cd274 using anti-PD-L1/Cd274 antibody (A00109-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1/Cd274 antigen affinity purified polyclonal antibody (Catalog # A00109-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1/Cd274 at approximately 40KD. The expected band size for PD-L1/Cd274 is at 40KD.
Protein Target Info & Infographic
Gene/Protein Information For Cd274 (Source: Uniprot.org, NCBI)
Gene Name
Cd274
Full Name
Programmed cell death 1 ligand 1
Weight
33275 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274;CD274;B7H1, PDCD1L1, PDCD1LG1, PDL1; CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 |PDCD1 ligand 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd274, check out the Cd274 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd274: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PD-L1/Cd274 Antibody Picoband® (A00109-3)
Hello CJ!
A00109-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
He J,Zhang W,Di T,Meng J,Qi Y,Li G,Zhang Y,Su H,Yan W.Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody.Biomed Pharmacother.2020
Species: Human,Mouse
A00109-3 usage in article: APP:WB, SAMPLE:HOS CELL AND K7M2 CELL, DILUTION:1:500
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