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Product Info Summary
| SKU: | PB9823 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TAP1 Antibody Picoband®
SKU/Catalog Number
PB9823
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TAP1 Antibody Picoband® catalog # PB9823. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9823)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TAP1, different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9823 is reactive to TAP1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
87 kDa
Calculated molecular weight
87218 MW
Background of TAP1
Transporter associated with Antigen Processing 1 is a protein that in humans is encoded by the TAP1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. And ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9823 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, HUT Whole Cell, SW620 Whole Cell
IHC: human intestinal cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TAP1 using anti-TAP1 antibody (PB9823).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate,
Lane 2: HUT Whole Cell Lysate,
Lane 3: SW620 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAP1 antigen affinity purified polyclonal antibody (Catalog # PB9823) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAP1 at approximately 87 kDa. The expected band size for TAP1 is at 87 kDa.
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Figure 2. IHC analysis of TAP1 using anti-TAP1 antibody (PB9823).
TAP1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TAP1 Antibody (PB9823) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TAP1 (Source: Uniprot.org, NCBI)
Gene Name
TAP1
Full Name
Antigen peptide transporter 1
Weight
87218 MW
Superfamily
ABC transporter superfamily
Alternative Names
Antigen peptide transporter 1;APT1;ATP-binding cassette sub-family B member 2;Peptide supply factor 1;Peptide transporter PSF1;PSF-1;Peptide transporter TAP1;Peptide transporter involved in antigen processing 1;Really interesting new gene 4 protein;TAP1;ABCB2, PSF1, RING4, Y3; TAP1 ABC17, ABCB2, APT1, D6S114E, PSF-1, PSF1, RING4*0102N, TAP1N, TAP1 transporter 1, ATP binding cassette subfamily B member peptide transporter 1|ABC transporter, MHC 1|ATP-binding cassette sub-family B member 2|ATP-binding cassette, sub-family B (MDR/TAP), member 2|peptide supply factor 1|peptide transporter PSF1|peptide transporter TAP1|peptide transporter involved in processing 1|really interesting new gene 4 protein|transporter 1 ATP-binding cassette sub-family B|transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)|transporter associated with processing|transporter, ATP-binding cassette, major histocompatibility complex, 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAP1, check out the TAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAP1 Antibody Picoband® (PB9823)
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1 Customer Q&As for Anti-TAP1 Antibody Picoband®
Question
We are currently using anti-TAP1 antibody PB9823 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
The anti-TAP1 antibody (PB9823) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-01
