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Product Info Summary
| SKU: | A08765 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-POLR2E (L58) Antibody
SKU/Catalog Number
A08765
Size
100ul
Form
Liquid
Description
Boster Bio Anti-POLR2E (L58) Antibody catalog # A08765. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLR2E (L58) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08765)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 30-80 of Human POLR2E.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08765 is reactive to POLR2E in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
24551 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08765 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of POLR2E using Anti-POLR2E (L58) Antibody (A08765).
Immunohistochemistry (IHC) analyzes of POLR2E (L58) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-POLR2E (L58) Antibody A08765
Western blot (WB) analysis of POLR2E (L58) polyclonal antibody at 1:500 dilutionLane1:CT26 whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:PC3 whole cell lysate(40ug)
Lane4:HCT116 whole cell lysate(40ug)
Lane5:786-O whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For POLR2E (Source: Uniprot.org, NCBI)
Gene Name
POLR2E
Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Weight
24551 MW
Superfamily
archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family
Alternative Names
DNA-directed RNA polymerases I, II, and III subunit RPABC1;RNA polymerases I, II, and III subunit ABC1;DNA-directed RNA polymerase II 23 kDa polypeptide;DNA-directed RNA polymerase II subunit E;RPB5 homolog;XAP4;POLR2E; POLR2E RPABC1, RPB5, XAP4, hRPB25, hsRPB5 RNA polymerase II, I and III subunit E DNA-directed RNA polymerases I, II, and III subunit RPABC1|DNA directed RNA polymerase II 23 kda polypeptide|DNA-directed RNA polymerase II 23 kDa polypeptide|DNA-directed RNA polymerase II subunit E|DNA-directed RNA polymerase subunit RPABC1|RNA polymerase II subunit E|RNA polymerases I, II, and III subunit ABC1|RPB5 homolog|polymerase (RNA) II (DNA directed) polypeptide E, 25kDa|polymerase (RNA) II subunit E
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR2E, check out the POLR2E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR2E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLR2E (L58) Antibody (A08765)
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No publications found for A08765
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