Anti-POLR2E (L58) Antibody

Product Name

Anti-POLR2E (L58) Antibody

View all POLR2E Antibodies

SKU/Catalog Number

A08765

Size

100ul

Form

Liquid

Description

Boster Bio Anti-POLR2E (L58) Antibody catalog # A08765. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-POLR2E (L58) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08765)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human SLC5A6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08765 is reactive to POLR2E in Human, Mouse, Rat

Applications

A08765 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

24551 MW

Background of POLR2E

Transports pantothenate, biotin and lipoate in the presence of sodium.

Wang H., J. Biol. Chem. 274:14875-14883(1999).
Prasad P.D., Arch. Biochem. Biophys. 366:95-106(1999).<

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of POLR2E using Anti-POLR2E (L58) Antibody (A08765).

Immunohistochemistry (IHC) analyzes of POLR2E (L58) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-POLR2E (L58) Antibody A08765

Western blot (WB) analysis of POLR2E (L58) polyclonal antibody at 1:500 dilution
Lane1:CT26 whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:PC3 whole cell lysate(40ug)
Lane4:HCT116 whole cell lysate(40ug)
Lane5:786-O whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For POLR2E (Source: Uniprot.org, NCBI)

Alternative Names

DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerases I, II, and III subunit RPABC1; hRPB25; hsRPB5; polymerase (RNA) II (DNA directed) polypeptide E (25kD); polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; RPABC1; RPB5 homolog; RPB5; XAP4RNA polymerases I, II, and III subunit ABC1 POLR2E RPABC1, RPB5, XAP4, hRPB25, hsRPB5 RNA polymerase II, I and III subunit E DNA-directed RNA polymerases I, II, and III subunit RPABC1|DNA directed RNA polymerase II 23 kda polypeptide|DNA-directed RNA polymerase II 23 kDa polypeptide|DNA-directed RNA polymerase II subunit E|DNA-directed RNA polymerase subunit RPABC1|RNA polymerase II subunit E|RNA polymerases I, II, and III subunit ABC1|RPB5 homolog|polymerase (RNA) II (DNA directed) polypeptide E, 25kDa|polymerase (RNA) II subunit E

For more info on POLR2E, check out the POLR2E Infographic

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In this infographic, you will see the following information for POLR2E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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