Product Info Summary
SKU: | A30655 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-RPAB1 Antibody
SKU/Catalog Number
A30655
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RPAB1 Antibody (Catalog# A30655). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPAB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30655)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RPAB1. Immunogen sequence location: 21-70
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30655 is reactive to POLR2E in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
24551 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30655 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from MCF-7 cells, using RPAB1 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For POLR2E (Source: Uniprot.org, NCBI)
Gene Name
POLR2E
Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Weight
24551 MW
Superfamily
archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family
Alternative Names
POLR2E;DNA-directed RNA polymerases I; II, and III subunit RPABC1;RNA polymerases I, II, and III subunit ABC1;DNA-directed RNA polymerase II 23 kDa polypeptide;DNA-directed RNA polymerase II subunit E;RPB5 homolog;XAP4 POLR2E RPABC1, RPB5, XAP4, hRPB25, hsRPB5 RNA polymerase II, I and III subunit E DNA-directed RNA polymerases I, II, and III subunit RPABC1|DNA directed RNA polymerase II 23 kda polypeptide|DNA-directed RNA polymerase II 23 kDa polypeptide|DNA-directed RNA polymerase II subunit E|DNA-directed RNA polymerase subunit RPABC1|RNA polymerase II subunit E|RNA polymerases I, II, and III subunit ABC1|RPB5 homolog|polymerase (RNA) II (DNA directed) polypeptide E, 25kDa|polymerase (RNA) II subunit E
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR2E, check out the POLR2E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR2E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPAB1 Antibody (A30655)
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No publications found for A30655
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-RPAB1 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart left ventricle using anti-RPAB1 antibody A30655. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
Does anti-RPAB1 antibody A30655 work for WB with heart left ventricle?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
According to the expression profile of heart left ventricle, POLR2E is highly expressed in heart left ventricle. So, it is likely that anti-RPAB1 antibody A30655 will work for WB with heart left ventricle.
Boster Scientific Support
Answered: 2019-07-15
Question
I see that the anti-RPAB1 antibody A30655 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-03
Question
We are currently using anti-RPAB1 antibody A30655 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
The anti-RPAB1 antibody (A30655) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-14
Question
Is this A30655 anti-RPAB1 antibody reactive to the isotypes of POLR2E?
G. Carter
Verified customer
Asked: 2017-12-18
Answer
The immunogen of A30655 anti-RPAB1 antibody is A peptide derived from human RPAB1. Immunogen sequence location: 21-70. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-18