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Product Info Summary
| SKU: | M08765 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-POLR2E Mouse Monoclonal Antibody [Clone ID: OTI3B5]
SKU/Catalog Number
M08765
Size
100 µl
Description
Boster Bio POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5). Catalog# M08765. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-POLR2E Mouse Monoclonal Antibody [Clone ID: OTI3B5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08765)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3B5
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human POLR2E (NP_002686) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08765 is reactive to POLR2E in Dog, Human, Monkey, Mouse, Rat
Applications
M08765 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either POLR2E (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2E antibody (M08765)
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Flow cytometric Analysis of Jurkat cells
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Anti-POLR2E mouse monoclonal antibody (M08765) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E.
Protein Target Info & Infographic
Gene/Protein Information For POLR2E (Source: Uniprot.org, NCBI)
Gene Name
POLR2E
Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Weight
Superfamily
archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family
Alternative Names
DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerases I, II, and III subunit RPABC1; hRPB25; hsRPB5; polymerase (RNA) II (DNA directed) polypeptide E (25kD); polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; RPABC1; RPB5 homolog; RPB5; XAP4RNA polymerases I, II, and III subunit ABC1 POLR2E RPABC1, RPB5, XAP4, hRPB25, hsRPB5 RNA polymerase II, I and III subunit E DNA-directed RNA polymerases I, II, and III subunit RPABC1|DNA directed RNA polymerase II 23 kda polypeptide|DNA-directed RNA polymerase II 23 kDa polypeptide|DNA-directed RNA polymerase II subunit E|DNA-directed RNA polymerase subunit RPABC1|RNA polymerase II subunit E|RNA polymerases I, II, and III subunit ABC1|RPB5 homolog|polymerase (RNA) II (DNA directed) polypeptide E, 25kDa|polymerase (RNA) II subunit E
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR2E, check out the POLR2E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR2E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLR2E Mouse Monoclonal Antibody [Clone ID: OTI3B5] (M08765)
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