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Product Info Summary
| SKU: | M00521 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Spastin Rabbit Monoclonal Antibody, Clone#RM346
SKU/Catalog Number
M00521
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Spastin Rabbit Monoclonal Antibody, Clone#RM346 (Catalog # M00521). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Spastin Rabbit Monoclonal Antibody, Clone#RM346 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00521)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM346
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human Spastin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Spastin.
Reactive Species
M00521 is reactive to SPAST in Human
Reconstitution
Calculated molecular weight
67197 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00521 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:500-1:1000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of lysate from HeLa cells, transfected with Control siRNA (1) or Spastin siRNA, using anti-Spastin rabbit monoclonal antibody (Clone RM346) at a 1:500 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human Brain tissue section using anti-Spastin rabbit monoclonal antibody (Clone RM346) at a 1:1000 dilution.
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Figure 3. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human Tonsil tissue section using anti-Spastin rabbit monoclonal antibody (Clone RM346) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SPAST (Source: Uniprot.org, NCBI)
Gene Name
SPAST
Full Name
Spastin
Weight
67197 MW
Superfamily
AAA ATPase family
Alternative Names
Spastin ;5.6.1.1 ;Spastic paraplegia 4 protein;SPAST ;ADPSP, FSP2, KIAA1083 , SPG4 ; SPAST ADPSP, FSP2, SPG4 spastin spastin|spastic paraplegia 4 (autosomal dominant; spastin)|spastic paraplegia 4 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPAST, check out the SPAST Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPAST: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Spastin Rabbit Monoclonal Antibody, Clone#RM346 (M00521)
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No publications found for M00521
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Customer Q&As
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5 Customer Q&As for Anti-Spastin Rabbit Monoclonal Antibody, Clone#RM346
Question
I see that the anti-Spastin Rabbit Monoclonal antibody, Clone#RM346 M00521 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-21
Question
I have a question about product M00521, anti-Spastin Rabbit Monoclonal antibody, Clone#RM346. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00521 anti-Spastin Rabbit Monoclonal antibody, Clone#RM346, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-25
Question
We are currently using anti-Spastin Rabbit Monoclonal antibody, Clone#RM346 M00521 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The anti-Spastin Rabbit Monoclonal antibody, Clone#RM346 (M00521) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-22
Question
Does anti-Spastin Rabbit Monoclonal antibody, Clone#RM346 M00521 work on monkey WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
Our lab technicians have not validated anti-Spastin Rabbit Monoclonal antibody, Clone#RM346 M00521 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Spastin Rabbit Monoclonal antibody, Clone#RM346 M00521 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-19
Question
I was wanting to use your anti-Spastin Rabbit Monoclonal antibody, Clone#RM346 for IHC for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
N. Carter
Verified customer
Asked: 2016-04-12
Answer
You can see on the product datasheet, M00521 anti-Spastin Rabbit Monoclonal antibody, Clone#RM346 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-12
