Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody

PLC-gamma 1 antibody

Boster Bio Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody catalog # M00677. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00677
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, ICC, WB

Product Name

Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody

View all PLC-gamma 1 Antibodies

SKU/Catalog Number

M00677

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody catalog # M00677. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00677)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FAA-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PLCG1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00677 is reactive to PLCG1 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

149 kDa

Calculated molecular weight

148532 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00677 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
ICC/IF 1:50-1:200
IP 1:50

Positive Control

WB: human Jurkat whole cell, human MCF-7 whole cell, human HepG2 whole cell, human SH-SY5Y whole cell, rat C6 whole cell, rat RH35 whole cell

Validation Images & Assay Conditions

Gene/Protein Information For PLCG1 (Source: Uniprot.org, NCBI)

Gene Name

PLCG1

Full Name

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

Weight

148532 MW

Alternative Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1;3.1.4.11;PLC-148;Phosphoinositide phospholipase C-gamma-1;Phospholipase C-II;PLC-II;Phospholipase C-gamma-1;PLC-gamma-1;PLCG1;PLC1; PLCG1 NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1 phospholipase C gamma 1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1|1-phosphatidyl-D-myo-inositol-4,5-bisphosphate|PLC-148|PLC-gamma-1|inositoltrisphosphohydrolase|monophosphatidylinositol phosphodiesterase|phosphatidylinositol phospholipase C|phosphoinositidase C|phosphoinositide phospholipase C|phosphoinositide phospholipase C-gamma-1|phospholipase C, gamma 1 (formerly subtype 148)|phospholipase C-148|phospholipase C-II|triphosphoinositide phosphodiesterase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PLCG1, check out the PLCG1 Infographic

PLCG1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PLCG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody

Question

We are currently using anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

The anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody (M00677) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-05-07

Question

I am interested in to test anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677 on human thymocyte for research purposes, then I may be interested in using anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

The products we sell, including anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-02

Question

Is a blocking peptide available for product anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody (M00677)?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

We do provide the blocking peptide for product anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody (M00677). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-04

Question

Would M00677 anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

You can see on the product datasheet, M00677 anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-03

Question

Can you help my question with product M00677, anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Anderson

Verified customer

Asked: 2018-09-18

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00677 anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymocyte using anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal antibody M00677. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-10-16

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-16

Order DetailsPrice
M00677

100μL

$370
M00677-30ul

30μL sample

$99
M00677-carrier-free-1ml

Carrier Free

$2590
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M00677
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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