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Product Info Summary
| SKU: | M00677-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PLCG1 Antibody (Center)
View all PLC-gamma 1 Antibodies
SKU/Catalog Number
M00677-1
Size
80 µl
Description
Boster Bio Anti-PLCG1 Antibody (Center) (Catalog # M00677-1). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PLCG1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00677-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PLCG1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 456-488 amino acids from the Central region of human PLCG1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00677-1 is reactive to PLCG1 in Human
Reconstitution
Calculated molecular weight
148532 Da
Background of PLC-gamma 1
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand- mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00677-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-PLCG1 Antibody (Center) at 1:2000 dilution
Lane 1: CCRF-CEM whole cell lysates
Lane 2: Hela whole cell lysates
Lane 3: MCF-7 whole cell lysates
Lane 4: Jurkat whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 149 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-PLCG1 Antibody (Center) at 1:2000 dilution
Lane 1: CCRF-CEM whole cell lysates
Lane 2: Hela whole cell lysates
Lane 3: HepG2 whole cell lysates
Lane 4: Jurkat whole cell lysates
Lane 5: MCF-7 whole cell lysates
Lane 6: PC-3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 149 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For PLCG1 (Source: Uniprot.org, NCBI)
Gene Name
PLCG1
Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Weight
148532 Da
Alternative Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, PLC-II, Phospholipase C-gamma-1, PLC-gamma-1, PLCG1, PLC1 PLCG1 NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1 phospholipase C gamma 1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1|1-phosphatidyl-D-myo-inositol-4,5-bisphosphate|PLC-148|PLC-gamma-1|inositoltrisphosphohydrolase|monophosphatidylinositol phosphodiesterase|phosphatidylinositol phospholipase C|phosphoinositidase C|phosphoinositide phospholipase C|phosphoinositide phospholipase C-gamma-1|phospholipase C, gamma 1 (formerly subtype 148)|phospholipase C-148|phospholipase C-II|triphosphoinositide phosphodiesterase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLCG1, check out the PLCG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLCG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLCG1 Antibody (Center) (M00677-1)
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