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Product Info Summary
| SKU: | PA1856 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Hyaluronidase-3 HYAL3 Antibody Picoband®
SKU/Catalog Number
PA1856
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hyaluronidase-3 HYAL3 Antibody catalog # PA1856. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hyaluronidase-3 HYAL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1856)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HYAL3, different from the related rat sequence by three amino acids, and different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1856 is reactive to HYAL3 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
93 kDa
Calculated molecular weight
46501 MW
Background of HYAL3
HYAL3 (hyaluronoglucosaminidase 3) is an enzyme that in humans is encoded by the HYAL3 gene, also known as LUCA3, Hyaluronidase-3, Lung carcinoma protein 3, LUCA-3, LUCA14, Minna14. By Northern blot analysis, expression of a 2.1-kb HYAL3 transcript that was strongest in testis and bone marrow, but relatively weak in other organs. HYAL3 encodes a predicted 417-amino acid protein. This gene encodes a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is though to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1856 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: 22RV Cell, HELA Cell, V20S Cell
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-HYAL3 antibody, PA1856, Western blotting
Lane 1: 22RV Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: V20S Cell Lysate
Click image to see more details
Anti-HYAL3 antibody, PA1856, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For HYAL3 (Source: Uniprot.org, NCBI)
Gene Name
HYAL3
Full Name
Hyaluronidase-3
Weight
46501 MW
Superfamily
glycosyl hydrolase 56 family
Alternative Names
Hyaluronidase-3;Hyal-3;3.2.1.35;Hyaluronoglucosaminidase-3;Lung carcinoma protein 3;LuCa-3;HYAL3;LUCA3; HYAL3 HYAL-3, LUCA-3, LUCA3 hyaluronidase 3 hyaluronidase-3|hyaluronoglucosaminidase 3|lung carcinoma protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HYAL3, check out the HYAL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HYAL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hyaluronidase-3 HYAL3 Antibody Picoband® (PA1856)
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No publications found for PA1856
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Hyaluronidase-3 HYAL3 Antibody Picoband®
Question
Is a blocking peptide available for product anti-HYAL3 antibody (PA1856)?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
We do provide the blocking peptide for product anti-HYAL3 antibody (PA1856). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-24
Question
We are currently using anti-HYAL3 antibody PA1856 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The anti-HYAL3 antibody (PA1856) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Is there a BSA free version of anti-HYAL3 antibody PA1856 available?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HYAL3 antibody PA1856 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-16
Question
Does PA1856 anti-HYAL3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
As indicated on the product datasheet, PA1856 anti-HYAL3 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-23
Question
My question regarding product PA1856, anti-HYAL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1856 anti-HYAL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-12
Question
I was wanting to use your anti-HYAL3 antibody for IHC for mouse bone marrow ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse bone marrow ovary identification?
N. Bhatt
Verified customer
Asked: 2019-01-23
Answer
It shows on the product datasheet, PA1856 anti-HYAL3 antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse bone marrow ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-23
Question
Does anti-HYAL3 antibody PA1856 work on goat WB with bone marrow ovary?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
Our lab technicians have not validated anti-HYAL3 antibody PA1856 on goat. You can run a BLAST between goat and the immunogen sequence of anti-HYAL3 antibody PA1856 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat bone marrow ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-03
