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Product Info Summary
| SKU: | A00677 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PLCG1 (Ab-771) Antibody
View all PLC-gamma 1 Antibodies
SKU/Catalog Number
A00677
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PLCG1 (Ab-771) Antibody (Catalog # A00677). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLCG1 (Ab-771) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00677)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized non-phosphopeptide derived from human PLCG1 around the phosphorylation site of tyrosine 771 (P-D-Y-G-A).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00677 is reactive to PLCG1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
148532 MW
Background of PLC-gamma 1
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Denis Tvorogov, J. Cell Sci., Feb 2005; 118: 601 - 610.
Brenda J. Irvin, Mol. Cell. Biol., Dec 2000; 20: 9149 - 9161.
Benoit Poulin, J. Biol. Chem., Feb 2000; 275: 6411.
Ichiro Nakamura, J. Cell Biol., Jan 2001; 152: 361.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00677 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using PLCG1 (Ab-771) antibody A00677.
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Western blot analysis of extracts from COS7 cells (Lane 2), and JK cells (Lane 3), using PLCG1 (Ab-771) antiobdy A00677. The lane on the left is treated with synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLCG1 (Ab-771) antibody A00677.
Protein Target Info & Infographic
Gene/Protein Information For PLCG1 (Source: Uniprot.org, NCBI)
Gene Name
PLCG1
Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Weight
148532 MW
Alternative Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1;3.1.4.11;PLC-148;Phosphoinositide phospholipase C-gamma-1;Phospholipase C-II;PLC-II;Phospholipase C-gamma-1;PLC-gamma-1;PLCG1;PLC1; PLCG1 NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1 phospholipase C gamma 1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1|1-phosphatidyl-D-myo-inositol-4,5-bisphosphate|PLC-148|PLC-gamma-1|inositoltrisphosphohydrolase|monophosphatidylinositol phosphodiesterase|phosphatidylinositol phospholipase C|phosphoinositidase C|phosphoinositide phospholipase C|phosphoinositide phospholipase C-gamma-1|phospholipase C, gamma 1 (formerly subtype 148)|phospholipase C-148|phospholipase C-II|triphosphoinositide phosphodiesterase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLCG1, check out the PLCG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLCG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLCG1 (Ab-771) Antibody (A00677)
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