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Product Info Summary
| SKU: | EK2002 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human LTBR ELISA Kit PicoKine®
View all Lymphotoxin beta R/TNFRSF3 ELISA kits
SKU/Catalog Number
EK2002
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LTBR ELISA Kit PicoKine® (96 Tests). Quantitate Human LTBR in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LTBR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2002)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q31-M227
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2002 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2002 is reactive to LTBR in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2002 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lymphotoxin beta R/TNFRSF3
Lymphotoxin beta receptor (LTBR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3), is a cell surface receptor for lymphotoxin involved in apoptosis and cytokine release. This gene is mapped to 12p13.31. This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2002-CAP | Anti-Human LTBR Pre-coated 96-well strip microplate | 1 |
| EK2002-ST | Human LTBR Standard | 2 vials, 10 ng/tube |
| EK2002-DA | Human LTBR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.037 | 0.063 | 0.143 | 0.285 | 0.523 | 1.037 | 1.678 | 2.412 |
Data Example Images
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Human LTBR PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lymphotoxin beta R/TNFRSF3, For serum, dilution ratio is 1:1 and concentration is around 300 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is around 60 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LTBR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 269 | 1216 | 3711 | 284 | 1234 | 4129 |
| Standard deviation | 11.03 | 57.15 | 181.84 | 14.48 | 67.87 | 260.13 |
| CV (%) | 4.1% | 4.7% | 4.9% | 5.1% | 5.5% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Lymphotoxin beta R/TNFRSF3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 269 | 279 | 260 | 274 | 270 | 7.01 | 2.5% |
| Sample 2 | 1216 | 1211 | 1144 | 1243 | 1203 | 36.44 | 3.0% |
| Sample 3 | 3711 | 4065 | 4020 | 3769 | 3891 | 153.46 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For LTBR (Source: Uniprot.Org, NCBI)
Gene Name
LTBR
Full Name
Tumor necrosis factor receptor superfamily member 3
Weight
46.709kDa
Alternative Names
Tumor necrosis factor receptor superfamily member 3; Lymphotoxin-beta receptor; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; Tumor necrosis factor receptor type III; TNF-RIII; TNFR-III; LTBR; D12S370; TNFCR; TNFR3; TNFRSF3 LTBR D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3 lymphotoxin beta receptor tumor necrosis factor receptor superfamily member 3|lymphotoxin B receptor|lymphotoxin beta receptor (TNFR superfamily, member 3)|tumor necrosis factor C receptor|tumor necrosis factor receptor 2-related protein|tumor necrosis factor receptor type III
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LTBR, check out the LTBR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LTBR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human LTBR ELISA Kit PicoKine® (EK2002)
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Customer Q&As
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5 Customer Q&As for Human LTBR ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I measured Lymphotoxin beta R level in serum.
Verified Customer
Verified customer
Asked: 2020-04-04
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-04-04
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Lymphotoxin beta R using the EK2002 Human Lymphotoxin beta R Picokine® ELISA Kit?
K. Jackson
Verified customer
Asked: 2019-08-22
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-08-22
Question
Q: What is the optimal O.D. value for Lymphotoxin beta R ELISA kit? I performed your Lymphotoxin beta R ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Lymphotoxin beta R even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-06-23
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Lymphotoxin beta R ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Lymphotoxin beta R that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-06-23
Question
Q: if the enzyme conjugated Lymphotoxin beta R antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Lymphotoxin beta R antigen?
Verified Customer
Verified customer
Asked: 2018-04-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-04-11
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
A. Johnson
Verified customer
Asked: 2016-02-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-02-27
