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Product Info Summary
| SKU: | M03911-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PKA R2 (PRKAR2A) Mouse Monoclonal Antibody [Clone ID: OTI1A7]
SKU/Catalog Number
M03911-2
Size
100 µl
Description
Boster Bio Anti-PRKAR2A (PKA R2 ) mouse monoclonal antibody, clone OTI1A7 (formerly 1A7). Catalog# M03911-2. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PKA R2 (PRKAR2A) Mouse Monoclonal Antibody [Clone ID: OTI1A7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03911-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1A7
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03911-2 is reactive to PRKAR2A in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
45.518kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03911-2 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Flow cytometric Analysis of Jurkat cells
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Anti-PRKAR2A mouse monoclonal antibody (M03911-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR2A.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and PRKAR2A-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A monoclonal antibody M03911-2 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Protein Target Info & Infographic
Gene/Protein Information For PRKAR2A (Source: Uniprot.org, NCBI)
Gene Name
PRKAR2A
Full Name
cAMP-dependent protein kinase type II-alpha regulatory subunit
Weight
45.518kDa
Superfamily
cAMP-dependent kinase regulatory chain family
Alternative Names
protein kinase, cAMP-dependent, regulatory, type II, alpha; RII-alpha subunit PRKAR2A PKR2, PRKAR2 protein kinase cAMP-dependent type II regulatory subunit alpha cAMP-dependent protein kinase type II-alpha regulatory subunit|cAMP-dependent protein kinase regulatory subunit RII alpha|protein kinase A, RII-alpha subunit|protein kinase, cAMP-dependent, regulatory subunit type II alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAR2A, check out the PRKAR2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKA R2 (PRKAR2A) Mouse Monoclonal Antibody [Clone ID: OTI1A7] (M03911-2)
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