Click image to see more details
Product Info Summary
| SKU: | FC00068-PE |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD40 Monoclonal Antibody PE Conjugated, Flow Validated
View all CD40/TNFRSF5 Antibodies
SKU/Catalog Number
FC00068-PE
Size
100 tests, 200 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD40 Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC00068-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD40 Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00068-PE)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: G28-5
Isotype
IgG1,k
Immunogen
Human tonsillar lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00068-PE is reactive to CD40 in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
30619 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00068-PE is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated PBMCs stained with PE conjugated anti-human CD40 (clone G28-5) (red histogram). Lymphocytes gated PBMCs stained with PE conjugated mouse IgG1 isotype control (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For CD40 (Source: Uniprot.org, NCBI)
Gene Name
CD40
Full Name
Tumor necrosis factor receptor superfamily member 5
Weight
30619 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5; CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD40, check out the CD40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD40 Monoclonal Antibody PE Conjugated, Flow Validated (FC00068-PE)
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Anti-Human CD40 Monoclonal Antibody PE Conjugated, Flow Validated
Question
My question regarding product FC00068-PE, anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00068-PE anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-30
Question
See below the WB image, lot number and protocol we used for left coronary artery using anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE. Please let me know if you require anything else.
B. Huang
Verified customer
Asked: 2019-12-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-20
Question
I am interested in to test anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE on human left coronary artery for research purposes, then I may be interested in using anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The products we sell, including anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-26
Question
I was wanting to use your anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated for Flow Cytometry for human left coronary artery on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human left coronary artery identification?
A. Evans
Verified customer
Asked: 2018-02-22
Answer
You can see on the product datasheet, FC00068-PE anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human left coronary artery in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-22
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left coronary artery using anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE. Let me know if you need anything else.
A. Patel
Verified customer
Asked: 2017-11-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-21
Question
Would FC00068-PE anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-14
Answer
As indicated on the product datasheet, FC00068-PE anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-14
Question
Would anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE work for Flow Cytometry with left coronary artery?
A. Johnson
Verified customer
Asked: 2017-02-14
Answer
According to the expression profile of left coronary artery, CD40 is highly expressed in left coronary artery. So, it is likely that anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE will work for Flow Cytometry with left coronary artery.
Boster Scientific Support
Answered: 2017-02-14
Question
Is this FC00068-PE anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated reactive to the isotypes of CD40?
L. Brown
Verified customer
Asked: 2016-12-13
Answer
The immunogen of FC00068-PE anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated is Human tonsillar lymphocytes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-12-13
Question
Do you have a BSA free version of anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE available?
F. Roberts
Verified customer
Asked: 2016-04-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-04-05
Question
Is a blocking peptide available for product anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated (FC00068-PE)?
G. Carter
Verified customer
Asked: 2016-03-22
Answer
We do provide the blocking peptide for product anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated (FC00068-PE). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-03-22
Question
I see that the anti-Human CD40 Monoclonal antibody PE Conjugated, Flow Validated FC00068-PE works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
A. Zhao
Verified customer
Asked: 2013-05-15
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-15
